Antibody-immobilized column for quick cell separation based on cell rolling

Biotechnology Progress
Atsushi Mahara, Tetsuji Yamaoka

Abstract

Cell separation using methodological standards that ensure high purity is a very important step in cell transplantation for regenerative medicine and for stem cell research. A separation protocol using magnetic beads has been widely used for cell separation to isolate negative and positive cells. However, not only the surface marker pattern, e.g., negative or positive, but also the density of a cell depends on its developmental stage and differentiation ability. Rapid and label-free separation procedures based on surface marker density are the focus of our interest. In this study, we have successfully developed an antiCD34 antibody-immobilized cell-rolling column, that can separate cells depending on the CD34 density of the cell surfaces. Various conditions for the cell-rolling column were optimized including graft copolymerization, and adjustment of the column tilt angle, and medium flow rate. Using CD34-positive and -negative cell lines, the cell separation potential of the column was established. We observed a difference in the rolling velocities between CD34-positive and CD34-negative cells on antibody-immobilized microfluidic device. Cell separation was achieved by tilting the surface 20 degrees and the increasing medium f...Continue Reading

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Citations

Apr 28, 2011·Biomicrofluidics·Huei-Wen WuGwo-Bin Lee
Jan 18, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Akihisa OtakaTetsuji Yamaoka

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