Antibody levels in goats fed Toxoplasma gondii oocysts

The Journal of Parasitology
S M NishiS M Gennari

Abstract

Outbred goats were fed 10(5) Toxoplasma gondii oocysts and were monitored twice weekly for 8 wk for rectal temperature, clinical signs, parasitemia, and antibody levels by indirect fluorescence antibody test (IFAT), latex agglutination test (LAT), and indirect enzyme-linked immunosorbent assay (ELISA). After 8 wk, all goats were killed, and samples of heart, skeletal muscle, brain, lymph nodes, kidneys, and liver were bioassayed in mice. Anorexia, fever, and lethargy were observed from day 3 to day 7 postinfection (PI). Parasitemia was detected by bioassay in 50% of infected goats from day 7 to day 14 PI. Viable T. gondii organisms were isolated from all infected goats. Antibodies to T. gondii were detected in some animals on day 10 PI by IFAT and LAT and on day 14 PI by ELISA. The infected goats were seropositive on day 17 PI.

References

Feb 1, 1976·Journal of Clinical Pathology·A VollerB Oladehin
Jul 15, 1989·Veterinary Parasitology·J P Dubey
Apr 6, 1999·Veterinary Parasitology·R W VitorB Fux

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Citations

Feb 9, 2013·The Journal of Parasitology·Flaviana Santos WanderleyRinaldo Aparecido Mota
Oct 15, 2010·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Luís Fernando SantanaCláudio Alessandro Massamitsu Sakamoto
May 23, 2014·The Journal of Veterinary Medical Science·Moizur RahmanYasuhiro Takashima

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA

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