Antibody-ligand interactions for hydrophobic charge-induction chromatography: a surface plasmon resonance study

Langmuir : the ACS Journal of Surfaces and Colloids
Fang ChengJing-Ping Qu

Abstract

This article describes the use of surface plasmon resonance (SPR) spectroscopy to study antibody-ligand interactions for hydrophobic charge-induction chromatography (HCIC) and its versatility in investigating the surface and solution factors affecting the interactions. Two density model surfaces presenting the HCIC ligand (mercapto-ethyl-pyridine, MEP) were prepared on Au using a self-assembly technique. The surface chemistry and structure, ionization, and protein binding of such model surfaces were characterized by X-ray photoelectron spectroscopy (XPS), near-edge X-ray absorption fine structure (NEXAFS), contact-angle titration, and SPR, respectively. The influences of the surface and solution factors, e.g., ligand density, salt concentration, and solution pH, on protein adsorption were determined by SPR. Our results showed that ligand density affects both equilibrium and dynamic aspects of the interactions. Specifically, a dense ligand leads to an increase in binding strength, rapid adsorption, slow desorption, and low specificity. In addition, both hydrophobic interactions and hydrogen bonding contribute significantly to the protein adsorption at neutral pH, while the electrostatic repulsion is overwhelmed under acidic cond...Continue Reading

References

Jul 20, 2002·Trends in Biotechnology·Egisto Boschetti
Jun 6, 2003·Journal of the American Chemical Society·Emily A SmithRobert M Corn
Aug 9, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Paul R MorrillChristopher R Lowe
Feb 3, 2004·Journal of Chromatography. a·Deborah K Follman, Robert L Fahrner
Oct 1, 2004·Chembiochem : a European Journal of Chemical Biology·Daniel M RatnerPeter H Seeberger
Feb 23, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Mandy YangHua-Zhong Yu
Apr 2, 2005·Biotechnology Progress·Sanchayita GhoseSteven M Cramer
Apr 20, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Thorsten BaunachDieter M Kolb
Sep 10, 2005·Nature Biotechnology·Anna M Wu, Peter D Senter
Nov 17, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Srinavya VutukuruRavi S Kane
Jan 10, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Kenan P FearsRobert A Latour
Jun 25, 2008·Journal of the American Chemical Society·Ilya LeventalPaul A Janmey
Sep 16, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Srinavya Vutukuru, Ravi S Kane
Oct 25, 2008·Journal of Chromatography. a·Guofeng ZhaoYan Sun
Oct 30, 2008·Analytical and Bioanalytical Chemistry·Jon LaddShaoyi Jiang
Jan 14, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Christophe SilienMichael Zharnikov
Sep 19, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Pranavan ThillaivinayagalingamAnthony R Newcombe
Nov 17, 2009·Protein Expression and Purification·Tsutomu ArakawaDaisuke Ejima
Feb 18, 2010·Journal of Electron Spectroscopy and Related Phenomena·J E BaioDavid G Castner
Apr 14, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Loren BaughDavid G Castner
Apr 22, 2010·Physical Chemistry Chemical Physics : PCCP·Jinxuan LiuChristof Wöll
Dec 15, 2010·Bioconjugate Chemistry·Fang ChengDaniel M Ratner
Jul 28, 2011·Physical Chemistry Chemical Physics : PCCP·Mutlu Iskender MuglaliMichael Rohwerder
Jan 10, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Jing ShangDaniel M Ratner
Jun 20, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Arthur YuDaniel M Ratner

❮ Previous
Next ❯

Citations

Oct 27, 2017·Current Protein & Peptide Science·Atsushi HiranoTomoshi Kameda
Nov 7, 2019·Nature Biomedical Engineering·Nimish GuptaShiladitya Sengupta
Jul 20, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jun RenLingyun Jia
Nov 23, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Mingyang LiQing Wang
Aug 15, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Seungjoo YiJi-Hun Seo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.