Antibody-mediated Hsp70 protein therapy

Brain Research
James E HansenRobert N Nishimura

Abstract

Intracellular Hsp70 provides cytoprotection against a variety of stressful stimuli, and an effective means of increasing intracellular Hsp70 levels could prove beneficial in the prevention and treatment of a variety of human diseases. A novel protein transduction domain consisting of the single chain Fv fragment of an anti-DNA antibody known to penetrate into living cells and tissues, mAb 3E10, has recently been used to deliver functional proteins to cells. The ability of the single chain Fv fragment to deliver Hsp70 into living cells was tested by generating an Fv-Hsp70 fusion protein. Fv-Hsp70 was produced as a secreted protein in both COS-7 cells and the methylotropic yeast strain Pichia pastoris and was shown capable of penetrating into COS-7 cells and primary rat cortical neurons. Pre-treatment with Fv-Hsp70 protected both COS-7 cells and primary rat cortical neurons against subsequent exposure to hydrogen peroxide. These results provide the first evidence that the Fv fragment of mAb 3E10 is capable of delivering proteins to neurons and indicate its potential in the development of Hsp70 protein therapy.

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Citations

Oct 27, 2012·Science Translational Medicine·James E HansenPeter M Glazer
Jan 16, 2010·Stroke; a Journal of Cerebral Circulation·Xinhua ZhanFrank R Sharp
Aug 28, 2012·Journal of Oral and Maxillofacial Pathology : JOMFP·Nutan TyagiAadithya B Urs
Jul 15, 2015·Scientific Reports·Richard H WeisbartJames E Hansen
Mar 25, 2016·Nature Reviews. Rheumatology·Philip W NobleJames E Hansen
May 7, 2016·Annals of the New York Academy of Sciences·Missag H ParseghianRichard A Richieri
Oct 28, 2016·Cell Stress & Chaperones·Yaroslav G GurskiyMichael B Evgen'ev
Nov 9, 2018·Frontiers in Pharmacology·Tatiana A SlastnikovaA S Sobolev
May 26, 2007·The Journal of Biological Chemistry·James E HansenRichard H Weisbart
Feb 6, 2017·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Haiqing YiBaodong Sun
Apr 3, 2015·Cancer Research·Philip W NobleJames E Hansen
Jul 30, 2019·Cell Metabolism·M Kathryn BrewerMatthew S Gentry
Sep 17, 2019·Trends in Molecular Medicine·Zhengqiu ZhouMatthew S Gentry
Feb 17, 2021·Toxicology Mechanisms and Methods·Stephen T HobsonMissag H Parseghian

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