Antibody-mediated neutralization of cytomegalovirus: modulation of efficacy induced through the IgG constant region

Molecular Immunology
Christina FurebringM Ohlin

Abstract

Antibodies can neutralize the infectious properties of human cytomegalovirus (CMV). In vivo, the major neutralization determinants are located on glycoprotein B (gB). Recombinant human antibodies, that carry different constant regions (IgG1, IgG3 and the synthetic variant IgG3mA) against two of these epitopes were investigated for their ability to recruit the complement cascade for destruction of the virus. It was shown that all variants of an antibody against the antigenic domain (AD)-2 epitope displayed a similar neutralization activity despite the fact that improved C1q binding was observed for IgG3 and IgG3mA over the IgG1 variant. In contrast, an antibody against the AD-1 epitope carrying the normal IgG3 constant region, was less efficient than its IgG1 counterpart in neutralizing the virus in the absence of complement. However, it restored its activity in the presence of complement to the level of the naturally occurring IgG1 version. The same antibody was substantially more potent in neutralizing the virus in the presence of complement if it carried the IgG3mA constant region. This demonstrates the importance of the constant domain for the biological activity of AD-1 specific antibodies, a factor that should be taken int...Continue Reading

References

Jun 1, 1992·Scandinavian Journal of Immunology·A C MalmborgC A Borrebaeck
Jul 1, 1988·The Journal of Experimental Medicine·C I BindonH Waldmann
Nov 1, 1987·The Journal of Experimental Medicine·M BrüggemannM S Neuberger
Dec 1, 1994·The Journal of General Virology·R R SpaeteM P Landini
Jul 1, 1996·European Journal of Immunology·O B SpillerD V Devine
Apr 8, 1998·Immunotechnology : an International Journal of Immunological Engineering·C FaberA B Edmundson
Apr 25, 2000·Virology·D R BurtonP W Parren

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Citations

Oct 16, 2003·Current Opinion in Pharmacology·O H Brekke, G Å Løset
Aug 17, 2006·Journal of Gastroenterology and Hepatology·Biplab BoseSubrata Sinha
Jul 15, 2004·International Immunopharmacology·Matthias BudtHartmut Hengel
Mar 25, 2015·Molecular Immunology·Mats Ohlin, Cecilia Söderberg-Nauclér
Mar 19, 2014·Current Opinion in Virology·Dai Wang, Tong-Ming Fu

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