Antibody-mediated versus nontargeted delivery in a human small cell lung carcinoma model

Bioconjugate Chemistry
E MarecosA Bogdanov

Abstract

The uptake of macromolecular agents in tumor cells (LX-1, human small cell lung carcinoma) and in corresponding tumor xenografts was compared in a parallel study utilizing a long-circulating biocompatible graft copolymer, MPEGs-PL-DTPA [Bogdanov, A., Jr., et al. (1995) Adv. Drug Delivery Rev. 16, 335-348; Bogdanov, A., Jr., et al. (1996) Bioconjugate Chem. 7, 144-149] and a tumor-specific chimeric monoclonal antibody, BR96 [Hellstrom, I., et al. (1990) Cancer Res. 50, 2183-2190; Garrigues, J., et al. (1993) Am. J. Pathol. 142, 607-622]. Covalent grafted conjugates of methoxy-(polyethylene glycol)succinate and polylysine and BR96 were modified with DTPA, biotinyl, or rhodamine-X-residues. Using radionuclide and fluorescent labeled derivatives of the copolymer and the antibody, we established that (1) the copolymer does not associate with the plasma membrane in N-ethylmaleimide-treated cells and is slowly internalized by live cells at 37 degrees C; (2) the antibody binds rapidly to the surface of LX-1 cells and shows active internalization in vesicles with a subsequent slow decrease in the cell-associated antibody concentration; (3) LX-1 cells bear more than 1 million BR96 binding sites/cell (with an apparent Kd of 4.5 x 10[-7] M...Continue Reading

References

Sep 1, 1979·The Journal of Cell Biology·J QuintartP Baudhuin
Apr 1, 1989·International Journal of Radiation Oncology, Biology, Physics·M J TrotterP L Olive
Jun 1, 1993·The Journal of Cell Biology·E L Racoosin, J A Swanson
Jan 1, 1996·Journal of Drug Targeting·G E FrancisA K Agrawal

❮ Previous
Next ❯

Citations

Aug 8, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·J KopecekC M Peterson
Jul 30, 1999·Journal of Controlled Release : Official Journal of the Controlled Release Society·M DvorákJ Kopecek
Jun 7, 2000·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·J KopecekZ Lu
Mar 30, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·S BredowR Weissleder
May 23, 2012·Journal of Biomedical Optics·Craig J GoergenAnand T N Kumar
Feb 8, 2011·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Doris GabrielNorbert Lange
Aug 9, 2011·Experimental and Molecular Pathology·Christine A St PierreRobert W Finberg
Jun 16, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Khaled GreishHiroshi Maeda
Dec 22, 1999·International Journal of Cancer. Journal International Du Cancer·M LewinR Weissleder
Apr 20, 1999·Nature Biotechnology·R WeisslederA Bogdanov
Aug 12, 2006·ChemMedChem·Yongdoo ChoiChing-Hsuan Tung
Jun 1, 2001·Nature Medicine·C BremerR Weissleder
Nov 17, 2007·Optics Letters·Vasilis NtziachristosRalph Weissleder
Dec 22, 1999·Journal of Biomedical Materials Research·M MikawaA Maruyama
Jan 20, 2004·Cancer Research·Khalid ShahRalph Weissleder
May 4, 2002·Neoplasia : an International Journal for Oncology Research·Alexei A BogdanovRalph Weissleder

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.