Antibody Production and Th1-biased Response Induced by an Epitope Vaccine Composed of Cholera Toxin B Unit and Helicobacter pylori Lpp20 Epitopes

Helicobacter
Yan LiYunshan Ning

Abstract

The epitope vaccine is an attractive potential for prophylactic and therapeutic vaccination against Helicobacter pylori (H. pylori) infection. Lpp20 is one of major protective antigens which trigger immune response after H. pylori invades host and has been considered as an excellent vaccine candidate for the control of H. pylori infection. In our previous study, one B-cell epitope and two CD4(+) T-cell epitopes of Lpp20 were identified. In this study, an epitope vaccine composed of mucosal adjuvant cholera toxin B subunit (CTB) and these three identified Lpp20 epitopes were constructed to investigate the efficacy of this epitope vaccine in mice. The epitope vaccine including CTB, one B-cell, and two CD4(+) T-cell epitopes of Lpp20 was constructed and named CTB-Lpp20, which was then expressed in Escherichia coli and used for intraperitoneal immunization in BALB/c mice. The immunogenicity, specificity, and ability to induce antibodies against Lpp20 and cytokine secretion were evaluated. After that, CTB-Lpp20 was intragastrically immunized to investigate the prophylactic and therapeutic efficacy in infected mice. The results indicated that the epitope vaccine CTB-Lpp20 possessed good immunogenicity and immunoreactivity and could e...Continue Reading

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Citations

Aug 18, 2016·Helicobacter·Dominique VelinMarkus Gerhard
Jun 13, 2019·Journal of Cellular Physiology·Masoud KeikhaMohsen Karbalaei
Oct 16, 2018·Frontiers in Immunology·Laura Antonio-HerreraLaura C Bonifaz
Jun 6, 2017·Critical Reviews in Microbiology·Nasrin MirzaeiHajieh Ghasemian Safaei
Nov 24, 2020·Frontiers in Cellular and Infection Microbiology·Jinling XieYan Li
Jun 18, 2021·Expert Review of Vaccines·Irineu Dos Santos VianaFabrício Freire de Melo
Sep 25, 2021·Critical Reviews in Microbiology·Roghayeh MohammadzadehHadi Farsiani

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