Antibody profiling and prevalence in US patients during the SARS-CoV2 pandemic

PloS One
Hari Krishnan KrishnamurthyJoseph A Murray

Abstract

Antibody diagnostics play an important role in disease detection and can potentially aid in monitoring of the immune responses to see if an individual has developed immunity. Developing high throughput diagnostics which does not involve handling of infectious material becomes imperative in the case of pandemics such as the recent outbreak of SARS-CoV2. A protein microarray technology was used to detect the plurality of antibody response to four novel antigens namely S1 glycoprotein, Receptor binding domain (RBD), S2 glycoprotein and Nucleoprotein of the novel coronavirus named SARS-CoV2 using serum samples. A DBS card was additionally used to compare its performance with a venipuncture-based serum separator tube (SST) draw. The three main subclasses of antibodies IgM, IgA and IgG were analyzed to see the variations in immune responses in the affected population and compared to their microbial RT-PCR based NP swab results. The clinical sensitivity and specificity were determined to be 99.67% and 99.77%. In the matrix comparison study, which would enable patients to test without risk of transmitting the virus, DBS (Dried Blood Spot) matched with higher than 98% accuracy to a venipuncture-based SST collection. Multiplex testing en...Continue Reading

References

Jan 25, 2020·The New England Journal of Medicine·Na ZhuUNKNOWN China Novel Coronavirus Investigating and Research Team
Jan 30, 2020·The New England Journal of Medicine·Qun LiZijian Feng
Feb 3, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Shi ZhaoMaggie H Wang
Feb 8, 2020·Clinical Chemistry·Y M Dennis Lo, Rossa W K Chiu
Mar 1, 2020·International Journal of Surgery·Catrin SohrabiRiaz Agha

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Methods Mentioned

BETA
PCR
ELISA
dried
blood spot
Antibody assay

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