Antibody reactivity to the transmembrane protein of the caprine arthritis encephalitis virus correlates with severity of arthritis: no evidence for the involvement of epitope mimicry

Veterinary Immunology and Immunopathology
J M DaviesP R Carnegie

Abstract

Serum and synovial antibody reactivities of caprine arthritis encephalitis virus (CAEV) infected goats were assessed by Western blotting against purified CAEV antigen and the greatest intensity of reactivity in the serum of arthritic goats was to the gp45 transmembrane protein (TM). The extracytoplasmic domain of the TM gene was cloned into a pGEX vector and expressed in Escherichia coli as a glutathione S transferase fusion protein (GST-TM). This clone was found to be 90.5 and 89.2% homologous to published sequences of CAEV TM gene. Serum of 16 goats naturally infected with CAEV were examined by Western blotting for reactivity to the fusion protein. Antibody reactivity to the GST-TM correlated with clinically detectable arthritis (R = 0.642, P < or = 0.007). The hypothesis that the immune response to the envelope proteins of the CAEV contributes to the severity of arthritis in goats naturally infected with CAEV via epitope mimicry was tested. Antibodies from 5 CAEV infected goats were affinity purified against the GST-TM fusion protein and tested for cross-reactivity with a series of goat synovial extracts and proteogylcans. No serum antibody response or cross-reactivity of affinity purified antibodies could be detected. Pepti...Continue Reading

References

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Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C DeLisi, J A Berzofsky
Sep 1, 1987·Arthritis and Rheumatism·K L BanksW P Cheevers
Jan 1, 1985·Archives of Virology·T M EllisW F Robinson
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Feb 1, 1986·International Journal for Parasitology·R M SandemanP R Carnegie
Jun 1, 1995·Clinical Immunology and Immunopathology·F SilvestrisF Dammacco
Apr 1, 1997·Immunology and Cell Biology·J M Davies

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