PMID: 9557740Apr 29, 1998Paper

Antibody response in mice inoculated with DNA expressing foot-and-mouth disease virus capsid proteins

Journal of Virology
J ChinsangaramM J Grubman

Abstract

Candidate foot-and-mouth disease (FMD) DNA vaccines designed to produce viral capsids lacking infectious viral nucleic acid were evaluated. Plasmid DNAs containing a portion of the FMDV genome coding for the capsid precursor protein (P1-2A) and wild-type or mutant viral proteinase 3C (plasmids P12X3C or P12X3C-mut, respectively) were constructed. Cell-free translation reactions programmed with pP12X3C (wild-type 3C) and pP12X3C-mut produced a capsid precursor, but only the reactions programmed with the plasmid encoding the functional proteinase resulted in P1-2A processing and capsid formation. Baby hamster kidney (BHK) cells also produced viral capsid proteins when transfected with these plasmids. Plasmid P12X3C was administered to mice by intramuscular, intradermal, and epithelial (gene gun) inoculations. Anti-FMD virus (FMDV) antibodies were detected by radioimmunoprecipitation (RIP) and plaque reduction neutralization assays only in sera of mice inoculated by using a gene gun. When pP12X3C and pP12X3C-mut were inoculated into mice by using a gene gun, both plasmids elicited an antibody response detectable by RIP but only pP12X3C elicited a neutralizing antibody response. These results suggest that capsid formation in situ i...Continue Reading

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Citations

Dec 16, 1998·Nature Biotechnology·C W Beard, P W Mason
Sep 27, 2003·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Qiaohua WuMarvin J Grubman
Apr 16, 2004·Clinical Microbiology Reviews·Marvin J Grubman, Barry Baxt
May 15, 2010·Journal of Biomedicine & Biotechnology·Penelope KorakaAlbert D M E Osterhaus
May 10, 2005·Immunology and Cell Biology·María José Dus Santos, Andrés Wigdorovitz
Mar 26, 2011·Expert Review of Vaccines·Luis L Rodriguez, Cyril G Gay
May 4, 2012·Expert Review of Vaccines·Veronica L Fowler, Paul V Barnett
Dec 29, 2012·Virologica Sinica·Raj Kishore NandaSubodh Kishore
Nov 10, 2014·Applied Microbiology and Biotechnology·Zhongwang ZhangYonglu Wang
May 29, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·V AzevedoS C Oliveira
Jan 4, 2017·Veterinary Microbiology·Fayna Diaz-San SegundoTeresa de Los Santos

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