Antibody response to immunization with purified GD3 ganglioside and GD3 derivatives (lactones, amide and gangliosidol) in the mouse

Immunobiology
G RitterP O Livingston

Abstract

GD3 is the ganglioside most abundantly expressed on the cell surface of human melanoma, and treatment with a monoclonal antibody recognizing GD3 has induced major responses in a small proportion of patients. However, we have been unable to induce production of GD3 antibodies in melanoma patients by active immunization with GD3-expressing melanoma cells or purified GD3. In this report we describe attempts to increase the immunogenicity of GD3 in the mouse by chemical modification. GD3 lactone I and II, GD3 amide and GD3 gangliosidol were synthesized, and the humoral immune response to these derivatives was compared with the response to unmodified GD3. The GD3 derivatives were more immunogenic than GD3. At a low dose all congeners induced an IgM response, with antibody titers higher than those elicited by low-dose GD3. The gangliosidol and amide derivatives also induced an IgG response. IgM antibodies induced by immunization with GD3 lactone I cross-reacted with purified GD3 and GD3-expressing melanoma cells. Titers of GD3 cross-reactive antibodies were slightly higher than after immunization with GD3 itself at the same low dose. IgM and IgG antibodies induced by the other congeners did not cross-react with GD3.

References

Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M PfreundschuhL J Old
Nov 1, 1987·Biological Chemistry Hoppe-Seyler·D KleinK Sandhoff
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P O LivingstonL J Old
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A N HoughtonL J Old
May 15, 1985·International Journal of Cancer. Journal International Du Cancer·T TaiD L Morton
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·L D CahanJ C Paulson

❮ Previous
Next ❯

Citations

May 1, 1994·Lipids·R Jennemann, H Wiegandt
Nov 14, 1997·Cancer Immunology, Immunotherapy : CII·P O Livingston, G Ragupathi
Dec 21, 1995·Brain Research. Developmental Brain Research·M L Allende, P Panzetta
Mar 15, 2006·Biochemistry·Peter ChefaloClifford V Harding
Feb 4, 2005·Journal of Medicinal Chemistry·Yanbin PanZhongwu Guo
Jul 9, 2004·Glycoconjugate Journal·Peter ChefaloZhongwu Guo
Apr 2, 2010·Nature Reviews. Drug Discovery·Rena D Astronomo, Dennis R Burton
Jul 22, 2005·Immunology and Cell Biology·Susan F SlovinGovind Ragupathi
Mar 10, 1994·Journal of Immunological Methods·M H RavindranathM C Graves
Feb 20, 2016·ACS Chemical Biology·Danyang FengFengshan Wang
Jul 29, 2004·Carbohydrate Research·Yanbin PanZhongwu Guo
Feb 16, 1999·The Journal of Investigative Dermatology·T TakahashiR F Irie
May 30, 1991·International Journal of Cancer. Journal International Du Cancer·G RitterP Livingston
Mar 4, 2000·International Journal of Cancer. Journal International Du Cancer·G RagupathiP O Livingston
Aug 12, 1993·Annals of the New York Academy of Sciences·P O Livingston
Mar 30, 2004·The Journal of Biological Chemistry·Wei ZouHarold J Jennings
Feb 20, 1993·International Journal of Cancer. Journal International Du Cancer·H BoeingJ Wahrendorf
Feb 20, 1993·International Journal of Cancer. Journal International Du Cancer·W B HamiltonP O Livingston
Apr 3, 2012·Journal of Carbohydrate Chemistry·Zhaojun Yin, Xuefei Huang
Feb 13, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Brian H KushnerNai-Kong V Cheung

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.