Antibody responses of calves to Histophilus somni recombinant IbpA subunits

Comparative Immunology, Microbiology and Infectious Diseases
Kimberly L LoL B Corbeil

Abstract

Histophilus somni causes bovine pneumonia and septicemia, but protective immune responses are not well understood and immunodiagnostic methods are not well defined. We previously showed that antibody to a new virulence factor, IbpA, neutralizes cytotoxicity and immunization with a recombinant IbpA domain protects calves against experimental H. somni pneumonia. To further define immune responses to IbpA, we determined isotypic serum antibody responses to three IbpA domains (IbpA3, an N-terminal coiled coil region; IbpA5, a central region of 200 bp repeats and IbpA DR2, a C-terminal cytotoxic domain). ELISA was used to quantitate IgG1 or IgG2 antibodies to each of the IbpA subunits as well as H. somni whole cells (WCs) or culture supernatant (SUP). Calves experimentally infected with H. somni and monitored for up to 10 weeks had the least "0 time" (background) antibody levels to IbpA5, as well as the earliest and highest responses of greatest duration to the IbpA5 subunit. Responses of these calves were high to WC or SUP antigens but with higher "0 time" (background) antibody levels. We concluded that IbpA5 may be a useful immunodiagnostic antigen. Calves immunized with H. somni WC vaccine had antibody responses to WC antigens, b...Continue Reading

References

Aug 1, 1988·Scandinavian Journal of Immunology·M YarnallL B Corbeil
Jul 1, 1988·Journal of General Microbiology·M YarnallL B Corbeil
Oct 6, 2004·Animal Health Research Reviews·Shivakumara Siddaramppa, Thomas J Inzana
Jul 11, 2006·Veterinary Immunology and Immunopathology·Lynette B CorbeilLaurel J Gershwin
Mar 10, 2009·Microbial Pathogenesis·Kaori HoshinooYuichi Tagawa
Apr 14, 2009·Molecular Cell·Carolyn A WorbyJack E Dixon

❮ Previous
Next ❯

Citations

Sep 14, 2012·Clinical and Vaccine Immunology : CVI·Carolina Guzmán-BrambilaDaniel Ortuño-Sahagún
Oct 16, 2015·Biologicals : Journal of the International Association of Biological Standardization·Claudia Avis MadampageAndrew Potter
Oct 2, 2012·Microbial Pathogenesis·Katrina M HellenbrandNicole A Aulik
Nov 5, 2016·The Veterinary Journal·Gerard M MurrayJoseph P Cassidy

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.