Abstract
Histophilus somni causes bovine pneumonia and septicemia, but protective immune responses are not well understood and immunodiagnostic methods are not well defined. We previously showed that antibody to a new virulence factor, IbpA, neutralizes cytotoxicity and immunization with a recombinant IbpA domain protects calves against experimental H. somni pneumonia. To further define immune responses to IbpA, we determined isotypic serum antibody responses to three IbpA domains (IbpA3, an N-terminal coiled coil region; IbpA5, a central region of 200 bp repeats and IbpA DR2, a C-terminal cytotoxic domain). ELISA was used to quantitate IgG1 or IgG2 antibodies to each of the IbpA subunits as well as H. somni whole cells (WCs) or culture supernatant (SUP). Calves experimentally infected with H. somni and monitored for up to 10 weeks had the least "0 time" (background) antibody levels to IbpA5, as well as the earliest and highest responses of greatest duration to the IbpA5 subunit. Responses of these calves were high to WC or SUP antigens but with higher "0 time" (background) antibody levels. We concluded that IbpA5 may be a useful immunodiagnostic antigen. Calves immunized with H. somni WC vaccine had antibody responses to WC antigens, b...Continue Reading
References
Jul 1, 1992·Molecular Microbiology·S P ColeL B Corbeil
Aug 1, 1988·Scandinavian Journal of Immunology·M YarnallL B Corbeil
Jul 1, 1988·Journal of General Microbiology·M YarnallL B Corbeil
Sep 1, 1993·Journal of General Microbiology·S P ColeL B Corbeil
Oct 6, 2004·Animal Health Research Reviews·Shivakumara Siddaramppa, Thomas J Inzana
Jun 28, 2005·Veterinary Immunology and Immunopathology·Laurel J GershwinLynette B Corbeil
Sep 20, 2005·Microbial Pathogenesis·Yuichi TagawaLynette B Corbeil
Jun 17, 2006·Vaccine·Londa J BerghausLaurel J Gershwin
Jul 11, 2006·Veterinary Immunology and Immunopathology·Lynette B CorbeilLaurel J Gershwin
Jul 2, 2008·Vaccine·Roger S GeertsemaLynette B Corbeil
Mar 10, 2009·Microbial Pathogenesis·Kaori HoshinooYuichi Tagawa
Apr 14, 2009·Molecular Cell·Carolyn A WorbyJack E Dixon
Feb 24, 2010·Infection and Immunity·Bereket ZekariasLynette B Corbeil
May 12, 2011·Vaccine·R S GeertsemaL B Corbeil
Citations
Sep 14, 2012·Clinical and Vaccine Immunology : CVI·Carolina Guzmán-BrambilaDaniel Ortuño-Sahagún
Oct 16, 2015·Biologicals : Journal of the International Association of Biological Standardization·Claudia Avis MadampageAndrew Potter
Oct 2, 2012·Microbial Pathogenesis·Katrina M HellenbrandNicole A Aulik
Aug 9, 2016·PloS One·Claudia Avis MadampageAndrew Potter
Nov 5, 2016·The Veterinary Journal·Gerard M MurrayJoseph P Cassidy
Aug 22, 2014·Clinical and Vaccine Immunology : CVI·Yu PanThomas J Inzana
Mar 10, 2017·Vaccine·Laurel J GershwinLynette B Corbeil