Antibody responses to bee melittin (Api m 4) and hornet antigen 5 (Dol m 5) in mice treated with the dominant T-cell epitope peptides

The Journal of Allergy and Clinical Immunology
T P KingH Agosto

Abstract

Mice treated with the dominant T-cell epitope peptides of allergens were reported to have reduced peptide or allergen-specific T-cell responses on subsequent immunization, but the extent of reduction of allergen-specific antibodies is not clear. This study was done to compare the extent of reduction of T-cell and antibody responses in peptide-treated mice. Two allergens were tested. Bee melittin (Api m 4), an allergen of 26 amino acid residues, has a single dominant T- or B-cell epitope. Hornet antigen 5 (Dol m 5), an allergen of 204 amino acid residues, has multiple dominant T- or B-cell epitopes. Mice were treated with T-cell peptides of Api m 4 or Dol m 5 and then immunized biweekly with their respective allergen with alum adjuvant. T-cell peptides tested were residues 7-19 of Api m 4 and residues 41-60, 141-160, and 176-195 of Dol m 5. T-cell responses at week 9 or 11 were assayed by proliferation of spleen cell cultures. Antibody responses of different isotypes were measured biweekly by ELISA. Partial reduction of 30% to 50% of T-cell responses to peptide or allergen was observed in bee and hornet peptide-treated mice. About 65% reduction of Api m 4-specific antibody response was observed early in the immune response but g...Continue Reading

Citations

Jun 15, 2007·Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology·Mark Larché
Apr 23, 2015·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·G BioleyB Corthésy
May 17, 2006·Immunology and Allergy Clinics of North America·Mark Larché
Feb 15, 2007·Allergy·M Larché
Apr 22, 2011·Allergy·D Moldaver, Mark Larché
Apr 23, 2015·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S R PrickettR E O'Hehir
Jun 13, 2017·Human Vaccines & Immunotherapeutics·Maximilian SchienerSimon Blank
May 16, 2017·Current Allergy and Asthma Reports·D Tomsitz, K Brockow
Apr 12, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J A AsturiasA Martínez

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