PMID: 9537039Jan 1, 1980Paper

Antibody secretion and DNA synthesis induced by lipopolysaccharide in enriched human lymphocyte subpopulations

Scandinavian Journal of Immunology
T Kunori, O Ringdén

Abstract

Lipopolysaccharide (LPS)-induced human lymphocyte activation was analysed with regard to DNA synthesis and antibody secretion. Antibody secretion was measured as plaque-forming cells (PFC) against fluorescein isothiocyanate (FITC)-coupled sheep erythrocytes (SRBC) or protein-A-coupled SRBC in the presence of developing antiserum against human IgG, IgA and IgM subclasses. By co-culturing lymphocytes with various concentrations of mitomycin, without impairing cell viability, inhibition of DNA synthesis and antibody secretion by LPS was correlated. Antibody secretion against FITC-SRBC or protein A-SRBC was not stimulated by LPS in B cells enriched by elimination of SRBC rosette-forming cells (E-RFC). In mixtures of enriched T and B cells the number of PFC was much lower than in unseparated lymphocytes, but the highest number of PFC was seen in the enriched E-RFC. However, DNA synthesis by LPS was induced in enriched B cells and not in enriched T cells. The highest DNA synthesis was seen for unseparated lymphocytes. Furthermore, autoradiography studies and experiments in which activated lymphocytes were separated revealed that LPS predominantly stimulated DNA synthesis in non-E-RFC, presumably B cells and only activated a small pro...Continue Reading

References

Jan 1, 1975·Scandinavian Journal of Immunology·O Ringeén, E Möller
Sep 1, 1975·Scandinavian Journal of Immunology·U Persson, E Möller
Jan 1, 1978·European Journal of Immunology·O Ringdén, Rynnel-Dagöö
Aug 1, 1976·European Journal of Immunology·E GronowiczF Melchers
Jan 1, 1975·Scandinavian Journal of Immunology·H LemkeE Gronowicz
Jan 1, 1975·Advances in Immunology·A Coutinho, G Möller
Jan 1, 1973·The Journal of Experimental Medicine·B S NilssonW W Bullock
Jan 1, 1972·Immunological Communications·S A EisenC W Parker
Mar 1, 1972·The Journal of Experimental Medicine·A BastenJ Pye
Sep 1, 1967·The Journal of Experimental Medicine·R I Mishell, R W Dutton
Jan 1, 1980·Scandinavian Journal of Immunology·O Ringdén

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