Antibody signature induced by SARS-CoV-2 spike protein immunogens in rabbits

Science Translational Medicine
Supriya RavichandranSurender Khurana

Abstract

Multiple vaccine candidates against SARS-CoV-2 based on viral spike protein are under development. However, there is limited information on the quality of antibody responses generated with these vaccine modalities. To better understand antibody responses induced by spike protein-based vaccines, we performed a qualitative study by immunizing rabbits with various SARS-CoV-2 spike protein antigens: S ectodomain (S1+S2; amino acids 16 to 1213), which lacks the cytoplasmic and transmembrane domains (CT-TM), the S1 domain (amino acids 16 to 685), the receptor binding domain (RBD) (amino acids 319 to 541), and the S2 domain (amino acids 686 to 1213, lacking the RBD, as control). Resulting antibody quality and function were analyzed by enzyme-linked immunosorbent assay (ELISA), RBD competition assay, surface plasmon resonance (SPR) against different spike proteins in native conformation, and neutralization assays. All three antigens (S1+S2 ectodomain, S1 domain, and RBD), but not S2, generated strong neutralizing antibodies against SARS-CoV-2. Vaccination-induced antibody repertoire was analyzed by SARS-CoV-2 spike genome fragment phage display libraries (SARS-CoV-2 GFPDL), which identified immunodominant epitopes in the S1, S1-RBD, an...Continue Reading

References

Feb 23, 2020·Science·Daniel WrappJason S McLellan
Mar 11, 2020·Cell·Alexandra C WallsDavid Veesler
Apr 8, 2020·Immunity·Fatima Amanat, Florian Krammer
Apr 11, 2020·Nature Reviews. Drug Discovery·Tung Thanh LeStephen Mayhew

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Citations

Aug 7, 2020·International Journal of Molecular Sciences·Sareh ZhandMark W Douglas
Dec 29, 2020·Advanced Drug Delivery Reviews·Jee Young ChungYoung Jik Kwon
Jan 12, 2021·The Journal of Biological Chemistry·Sameer Kumar MalladiRaghavan Varadarajan
Dec 15, 2020·Current Opinion in Biotechnology·Christiaan R de VriesPaul L Bollyky
Dec 21, 2020·Stem Cell Research·Shi XiaojieQiang Min
Dec 20, 2020·Nature Reviews. Immunology·Lianpan Dai, George F Gao
Jan 16, 2021·Science·Brian HieBryan Bryson
Jan 5, 2021·Genome Génome / Conseil National De Recherches Canada·Rwik SenYanan Lu
Feb 16, 2021·Protein Science : a Publication of the Protein Society·Benjamin N BellPeter S Kim
May 1, 2021·Frontiers in Molecular Biosciences·Xiaohui ZhaoHongliang Wang
May 22, 2021·Viral Immunology·Noha A KamelKhaled M Aboshanab
May 14, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xiaojuan LiuWei Cun
Dec 10, 2020·ACS Infectious Diseases·Zifang ShangWei Huang
Jun 25, 2021·PLoS Pathogens·Min ZhaoUNKNOWN Yale IMPACT Research Team
Jun 27, 2021·Science Advances·Jacob T HeggestadAshutosh Chilkoti
Jul 4, 2021·Journal of Hazardous Materials·Ives Charlie-SilvaGuilherme Malafaia
Jul 1, 2021·Oxford Open Immunology·D Oliver ScourfieldStephanie E A Burnell
Jul 3, 2021·Viruses·Esen SokulluBenoit Coulombe
Jul 20, 2021·International Immunopharmacology·Yuval IshayFadi Kharouf
Apr 17, 2021·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Juanjie TangSurender Khurana

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Datasets Mentioned

BETA
MN908947
AAP30030.1
AKN11075.1
AVP78031.1
AVP78042.1
ADK66841.1
AFS88936.1

Methods Mentioned

BETA
phage display
surface plasmon resonance
chips
ELISA
chip
glycosylation
transfection

Software Mentioned

Rad ProteOn Manager
BioRad ProteOn manager
Bio

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