PMID: 108667Mar 1, 1979

Antibody to B. subtilis DNA polymerase III: use in enzyme purification and examination of homology among replication-specific DNA polymerases

Nucleic Acids Research
M H Barnes, N C Brown

Abstract

Bacillus subtilis DNA polymerase III (pol III), an arylhydrazinopyrimidine-sensitive, replication-specific enzyme, was used to generate a non-precipitating rabbit antibody which specifically inhibited pol III activity in vitro. The antibody was used to examine structural relationships among several DNA polymerases, and it was linked covalently to agarose; the antibody:agarose was employed to develop a rapid, selective method of purification of catalytically active B. subtilis pol III.

References

Jan 1, 1975·Annual Review of Biochemistry·M L Gefter
Dec 1, 1973·Analytical Biochemistry·W Schaffner, C Weissmann
Jun 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·E Lazarides, K Weber
Nov 15, 1972·Nature: New Biology·G W Bazill, J D Gross
Jan 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·K B GassN R Cozzarelli
Feb 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J M MackenzieN C Brown
Jan 1, 1968·Cold Spring Harbor Symposia on Quantitative Biology·B M AlbertsF L Ferris

Citations

Jan 1, 1990·Pharmacology & Therapeutics·G E Wright, N C Brown
Feb 17, 2006·Journal of Medicinal Chemistry·C ZhiRichard Storer
Nov 1, 2006·Antimicrobial Agents and Chemotherapy·Michelle ButlerTerry L Bowlin
Jan 25, 2005·Bioorganic & Medicinal Chemistry Letters·George E WrightMichelle Butler
Feb 1, 1992·Protein Expression and Purification·R A Hammond, N C Brown
Oct 15, 1986·Biochemical and Biophysical Research Communications·K A FosterU Hübscher
Jun 14, 2000·The Journal of Biological Chemistry·N KlempererM O'Donnell
Jul 6, 2000·The Journal of Biological Chemistry·I Bruck, M O'Donnell

Related Concepts

Antigen-Antibody Reactions
Natto Bacteria
DNA Polymerase III
DNA Polymerase N3
DNA Replication

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