Antibody to mCLCA3 suppresses symptoms in a mouse model of asthma

PloS One
Li-qiang SongYan Li

Abstract

Asthma is a complex and heterogeneous chronic inflammatory disorder that is associated with mucous cell metaplasia and mucus hypersecretion. Functional genomic analysis indicates that mucous cell metaplasia and mucus hypersecretion depend on members of the calcium-activated chloride channel (CLCA) gene family. It has been reported that the inhibition of CLCAs could relieve the symptoms of asthma. Thus, the mCLCA3 antibody may be a promising strategy to treat allergic diseases such as asthma. We constructed asthmatic mouse models of OVA-induced chronic airway inflammatory disorder to study the function of the mCLCA3 antibody. Airway inflammation was measured by HE staining; goblet cell hyperplasia and mucus hypersecretion were detected by PAS staining; muc5ac, IL-13, IFN-γ levels in bronchoalveolar lavage fluid (BALF) were examined by ELISA; Goblet cell apoptosis was measured by TUNEL assay and alcian blue staining; mCLCA3, Bcl-2 and Bax expression were detected by RT-PCR, Western blotting and immunohistochemical analysis. In our study, mice treated with mCLCA3 antibody developed fewer pathological changes compared with control mice and asthmatic mice, including a remarkable reduction in airway inflammation, the number of goblet...Continue Reading

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Citations

Nov 28, 2015·Mediators of Inflammation·Monica Sala-RabanalTom J Brett
Nov 10, 2015·Molecular Medicine Reports·Zhen-Hua NiXiong-Biao Wang
Jan 17, 2017·Mediators of Inflammation·Giuseppe SpazianoBruno D'Agostino

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Methods Mentioned

BETA
ELISA
reverse transcription-PCR
PCR
protein assay
electrophoresis
confocal microscopy

Software Mentioned

SPSS17

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