PMID: 11340639May 8, 2001Paper

Antibody to the extracellular domain of the low affinity NGF receptor stimulates p75(NGFR)-mediated apoptosis in cultured sympathetic neurons

Journal of Neuroscience Research
M M Freidin

Abstract

Recent evidence has established a role for p75(NGFR) in developmentally regulated neuronal cell death. Although cell death due to NGF withdrawal is a well described, apoptosis in sympathetic neurons through stimulation of p75(NGFR) has not been clearly demonstrated. We have found that an antibody directed against the extracellular domain of murine p75(NGFR) profoundly effects the survival of short-term cultures of sympathetic neurons. Rat superior cervical ganglion neurons grown in the presence of NGF and treated with the bioactive antibody (9651) display a dose-dependent increase in cell death. This effect was independent of NGF concentration and partially reversed by either depolarizing stimuli or forskolin. The response to 9651 seems to act directly through a p75(NGFR)-mediated pathway and not by disturbing p75(NGFR)/TrkA interactions. Moreover, the kinetics of antibody stimulated cell death was more rapid than the cell death resulting from removal of NGF and treatment with CNTF failed to promote neuronal survival in the presence of 9651. Initiation of cell death is often associated with decreased NFkappaB activity, whereas survival or rescue correlates with increased NFkappaB. Increases in NFkappaB, however, have been obser...Continue Reading

References

Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M Freidin, J A Kessler
Jul 2, 1993·Regulatory Peptides·J A KesslerK J Chandross
May 28, 1993·Annals of the New York Academy of Sciences·T L Deckwerth, E M Johnson
Feb 1, 1995·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·P RaoM V Chao
Jun 19, 1997·Nature·N L LillD M Livingston
Jun 27, 1997·Cell·M L AvantaggiatiK Kelly
Aug 1, 1997·Neurochemistry International·J Y Chang, V V Korolev
Oct 30, 1998·Cellular Signalling·J R Orlinick, M V Chao
Jan 16, 1999·The Journal of Biological Chemistry·R WadgaonkarT Collins
Jan 23, 1999·The Journal of Biological Chemistry·G KhursigaraM V Chao
Jun 9, 1999·Current Biology : CB·L P KaneA Weiss

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis