Anticancer activity of Ashwagandha against human head and neck cancer cell lines

Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
Haeng-Eun LeeSung-Dae Cho

Abstract

The aim of this study was to determine the apoptotic activity of methanol extract of Ashwagandha (MEAG) and in human head and neck squamous cell carcinoma (HNSCC) cells and to investigate the underlying mechanisms. We investigated the effects of MEAG on programmed cell death in HNSCC cells using a Live/Dead assay, detection of nuclear morphologic changes, Mitotracker, siRNA knockdown, and RT-PCR. Treatment with MEAG showed dose-dependent growth-inhibitory activity that attribute to caspase-dependent apoptosis. Loss of mitochondrial membrane potential, release of cytochrome c, and activation of caspase 9 suggested that MEAG leads to activation of mitochondria-mediated apoptosis. MEAG selectively upregulated the expression of Bim protein at the transcriptional level and induced the translocation of Bim into the mitochondria. Knockdown of Bim by siRNA partially blocked MEAG-mediated apoptosis. MEAG also caused an increase in truncated Bid (t-Bid), cleaved caspase-8, and death receptor 5 (DR5). Interestingly, withaferin A (WA), a bioactive component of MEAG, clearly induced apoptosis accompanied by upregulation of Bim, t-Bid, caspase-8, and DR5 similar to the effects of MEAG. These suggest that MEAG and WA may be potential natural ...Continue Reading

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Citations

Mar 10, 2016·International Journal of Molecular Sciences·In-Chul Lee, Bu Young Choi
May 17, 2018·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Dong-Hoon WonSung-Dae Cho
Nov 23, 2017·Aging·Alexander AliperAlex Zhavoronkov
Oct 19, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Su-Jung ChoiSung-Dae Cho
Mar 17, 2020·Journal of Ethnopharmacology·Akash SaggamBhushan Patwardhan
Apr 18, 2021·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Abedul HaqueArm R Amin

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