Anticancer activity of Bombyx batryticatus ethanol extract against the human tumor cell line HeLa

Genetics and Molecular Research : GMR
W P WuD Wang

Abstract

Anticancer activity of Bombyx batryticatus ethanol extract (BBE) against HeLa cells was studied using cell viability, DNA fragmentation, real-time polymerase chain reaction, and Western blot analyses. The BBE inhibited the growth and induced apoptosis of HeLa cells. The MTT assay indicated that the BBE induced cytotoxicity in HeLa cells in a time- and concentration-dependent manner. When HeLa cells were treated for 48 h, the 50% inhibitory concentration (IC₅₀) value for the BBE was 1.564 mg/mL. The microscopy results showed that HeLa cells were severely distorted and showed slow growth; some cells became round in shape when treated with 5 mg/mL BBE for 24 h. The DNA ladder results revealed excessive DNA fragmentation in HeLa cells treated with 7 mg/mL BBE for 36 h. The proapoptotic activity of the BBE was attributed to its ability to modulate the expression of Bcl-2 and Bax genes. The mRNA and protein expression levels of Bax were remarkably higher whereas those of Bcl-2 were lower than those in the control cells; this led to an increased Bax/Bcl-2 ratio in cells treated with the BBE for 36 h. The results suggest that the BBE might play an important role in tumor growth suppression by inducing apoptosis in human cervical cancer...Continue Reading

Citations

Oct 21, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Meibian HuChunjie Wu
Oct 26, 2018·Journal of Cellular and Molecular Medicine·Jun LinWeiwen Cai
Jan 23, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ha-Yeon SongEui-Baek Byun

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