Anticancer activity of genistein on implanted tumor of human SG7901 cells in nude mice.

World Journal of Gastroenterology : WJG
Hai-Bo ZhouChan-Ni Wu

Abstract

To investigate genistein-induced apoptosis of implanted tumors of SG7901 cells in nude mice, and the relationship between this apoptosis and expression of Bcl-2 and Bax. Establishing a transplanted tumor model by injecting human SG7901 cells into subcutaneous tissue of nude mice. Genistein (0.5, 1 and 1.5 mg/kg) was directly injected adjacent to the tumor, six times at 2-d intervals. Then, changes in tumor volume were measured continuously and tumor inhibition rate of each group was calculated. We observed the morphological alterations by transmission electron microscopy (TEM), measured the apoptotic rate by the TUNEL staining method, and detected the expression of apoptosis-regulated gene Bcl-2 and bax by immunohistochemical staining and RT-PCR. Genistein 0.5, 1 and 1.5 mg/kg significantly inhibited carcinoma growth when it was injected near the tumor by 10.8%, 29.9% and 39.6%, respectively. Genistein induced implanted tumor cells to undergo apoptosis, with apoptotic characteristics seen by TEM. The apoptosis index was increased progressively with increasing genistein dose (28.9%+/-1.2%, 33.8%+/-1.6% and 37.7%+/-1.2%). The positive rate of Bcl-2 protein was decreased progressively (11.9%+/-0.9%, 5.9%+/-0.7% and 4.2%+/-0.6%), a...Continue Reading

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Citations

Oct 21, 2009·World Journal of Gastroenterology : WJG·Yan GuBo Sun
Oct 28, 2009·World Journal of Gastroenterology : WJG·Chun-Gen XingZheng-Hong Qin
Nov 5, 2014·Critical Reviews in Food Science and Nutrition·Mohammad F UllahAamir Ahmad
Jul 3, 2013·Journal of Epidemiology·Kwang-Pil KoKeun-Young Yoo
Dec 15, 2017·The Journal of International Medical Research·Shu WangQi Zhang
Mar 29, 2011·Critical Reviews in Toxicology·Susanne AndresAlfonso Lampen
Sep 18, 2008·The Journal of Clinical Endocrinology and Metabolism·Herbert MariniFrancesco Squadrito
Jul 17, 2015·Advances in Nutrition·Carmela SpagnuoloSeyed Mohammad Nabavi
May 8, 2010·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Kwang-Pil KoKeun-Young Yoo

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