Anticancer Activity of Metformin, an Antidiabetic Drug, Against Ovarian Cancer Cells Involves Inhibition of Cysteine-Rich 61 (Cyr61)/Akt/Mammalian Target of Rapamycin (mTOR) Signaling Pathway.

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Fengli ZhangLixiao Yang

Abstract

BACKGROUND Ovarian cancer is considered one of the lethal cancers responsible for high mortality and morbidity across the world. The prognosis and the survival rate of ovarian cancer is far from decent. Cysteine-rich 61 (Cyr61) also known as CCN1, is a member of CCN-family of growth factors, reported to be significantly overexpressed in several malignancies which include, but are not limited to, ovarian cancer. Recent studies have revealed that women with type 2 diabetes mellitus have an elevated risk of ovarian cancer. Hence, administration of an antidiabetic drug with anticancer effects such as metformin may act as an effective therapeutic regime against ovarian cancer. MATERIAL AND METHODS Cell viability and apoptosis were examined by MTT and Annexin V/PI double staining respectively. Cell migration was determined by Boyden Chamber assay. Transient knockdown of Cyr61 in ovarian cancer cells was achieved by transecting the cells with siRNA for Cyr61using Lipofectamine 2000. RESULTS Our results indicated that treatment of ovarian cancer cells with metformin caused significant downregulation of Cyr61 protein expression levels ultimately favoring apoptosis. Transient knockdown of Cyr61 resulted in the inhibition of cell prolifer...Continue Reading

Citations

Jul 25, 2019·Current Pharmaceutical Design·Yongbo WangZhi-Jiang Zhang
Dec 2, 2020·International Journal of Molecular Sciences·Agnieszka KosowskaTomasz Francuz
Apr 21, 2021·Journal of Cell Communication and Signaling·Herman Yeger, Bernard Perbal
Aug 28, 2021·Biomedicines·Santosh Kumar SinghRajesh Singh

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Methods Mentioned

BETA
ELISA
transfection

Software Mentioned

GraphPad Prism
GraphPad
FACS Diva

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