PMID: 16619490Apr 20, 2006Paper

Anticancer activity of MS-275, a novel histone deacetylase inhibitor, against human endometrial cancer cells

Anticancer Research
Noriyuki TakaiHisashi Narahara

Abstract

Histone deacetylase inhibitors (HDACIs) can inhibit cell proliferation, induce cell cycle arrest and stimulate apoptosis of cancer cells. The effects of a novel HDACI, MS-275, on 4 endometrial cancer cell lines and normal human endometrial epithelial cells was investigated. Endometrial cancer cells were treated with various concentrations of MS-275 and its effect on cell growth, cell cycle, apoptosis and related measurements was investigated. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays showed that all endometrial cancer cell lines were sensitive to the growth inhibitory effect of MS-275, although the normal endometrial epithelial cells were viable after treatment with the same doses of MS-275 that induced growth inhibition in endometrial cancer cells. The cell cycle analysis indicated that their exposure to MS-275 induced the G0/G1 arrest of the cell cycle. Induction of apoptosis was confirmed by annexin V staining of externalized phosphatidylserine and loss of the transmembrane potential of mitochondria. This induction occurred in concert with altered expression of genes related to cell growth, malignant phenotype and apoptosis. These results raise the possibility that MS-275 may prove particularly ...Continue Reading

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.