Anticancer activity of tetrandrine by inducing pro-death apoptosis and autophagy in human gastric cancer cells

The Journal of Pharmacy and Pharmacology
Xin-Yu BaiPing Liu

Abstract

To investigate the antitumour property of tetrandrine by inducing autophagy and apoptosis in human gastric cancer cells, and to explore the potential molecular mechanisms. The antitumour activity of tetrandrine was assessed through MTT assay. Apoptosis was measured by flow cytometry and microscopic examination of cellular morphology. The mitochondrial membrane potential was detected by staining with Rh-123. Induction of autophagy was monitored by transmission electron microscopy observation, using GFP-LC3 transfection. The results revealed that tetrandrine exhibits significant antitumour activity against gastric human cancer cell and the antigastric tumour activity was depended on inducing autophagy and apoptosis through upregulating the apoptosis-related protein (cleaved PARP, cleaved caspase-3 and cleaved caspase-9) and autophagy-related protein (Beclin-1, LC3-II and p62), and decreasing the phosphorylation of AKT/mTOR, PS6K and P-4EBP1. Adding the inhibitor of autophagy, 3-MA or Baf-A1, increased the viability of tetrandrine-exposed gastric cancer cells, which confirmed the role of autophagy played in the gastric cancer cell death induced by tetrandrine. These results demonstrated that the antitumour effects of tetrandrine b...Continue Reading

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Citations

Nov 23, 2018·Naunyn-Schmiedeberg's Archives of Pharmacology·Amira SalehWaleed Barakat
May 3, 2019·The Journal of Pharmacy and Pharmacology·Ziyan GuoJianqing Yu
Apr 28, 2020·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Yueping JiangShao Liu
Nov 11, 2020·International Journal of Molecular Sciences·Sylwia NakoniecznaWirginia Kukula-Koch
Dec 10, 2020·Cancer Management and Research·Wen-Jie HuangPeng Shu
May 13, 2021·International Journal of General Medicine·Ting XiuFan-Bo Jing
May 27, 2021·Phytotherapy Research : PTR·Liang LyuXiujie Wang

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