Anticancer and apoptotic activities of parthenolide in combination with epirubicin in mda-mb-468 breast cancer cells.

Molecular Biology Reports
Arash Ghorbani-Abdi-SaedabadReyhane Hoshyar

Abstract

Breast cancer is the most common malignancy in women worldwide. Unfortunately, current therapeutic methods are not completely efficient. Hence, combination therapy with medicinal plants has attracted several kinds of research. In the current study, we aimed to investigate the apoptotic and anti-cancer effect of Parthenolide in combination with Epirubicin in the MDA-MB-468 breast cancer cell line. In this study,  the anti-proliferative and pro-apoptotic effect of Parthenolide in combination with Epirubicin and without it, in the MDA-MB-468 cell line have been assessed by MTT test, Hoescht staining and flow cytometry methods. Our outcomes showed that Parthenolide treatment in the present of Epirubicin led to a decrease in the minimum toxic concentration of Parthenolide and Epirubicin in comparison with individual treatments. Then, to achieve a likely molecular mechanism of mentioned drugs Bax and Bcl2 expression level evaluated by Real-time PCR and subsequently, Western blotting has been estimated the protein level of Caspase 3. Our data indicated that the treatment of cells with Parthenolide led to up-regulation of Bax and downregulation of Bcl2 at mRNA level. Moreover, Parthenolide treatment led to the obvious alternation of Ca...Continue Reading

References

Sep 19, 2006·The International Journal of Biochemistry & Cell Biology·Marian ValkoJoshua Telser
Jun 15, 2007·Toxicologic Pathology·Susan Elmore
Jun 11, 2010·Annals of Oncology : Official Journal of the European Society for Medical Oncology·I HarirchiM Ebrahimi
Oct 23, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jin Dai, Russell J Mumper
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
Aug 11, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Guang Cheng, Liping Xie
Jul 17, 2012·Anti-cancer Drugs·Maria Regina Orofino KreugerKatharina D'Herde
Jul 8, 2014·Bioorganic & Medicinal Chemistry Letters·Mohamed MoumouMohamed Akssira
Dec 9, 2014·Biochemical and Biophysical Research Communications·Bei LanYoujia Cao
Dec 17, 2014·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Mohammad Yahya Hanafi-BojdBizhan Malaekeh-Nikouei
Sep 8, 2015·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Norihiro YamaguchiRonald H Blum
Apr 3, 2016·Cancer Letters·Sahdeo PrasadAmit K Tyagi
Jul 18, 2017·Nature Communications·Akane Inoue-YamauchiEmily H Cheng
Aug 2, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Homa MollaeiReyhane Hoshyar
Mar 10, 2018·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Nesa JafariSattar Tahmasebi Enferadi
Apr 27, 2018·Biomaterials·Si-Yong QinXian-Zheng Zhang
Jun 21, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Man LiuQiang Yu
Sep 28, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Wamidh H Talib, Lina T Al Kury
Nov 26, 2019·Natural Product Reports·Robert R A FreundHans-Dieter Arndt
Mar 11, 2020·Expert Review of Clinical Pharmacology·Edward R Sauter

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Citations

Aug 20, 2019·Current Medicinal Chemistry·Thaise Gonçalves AraújoAdemar Alves da Silva Filho
Jul 18, 2021·Medical Oncology·Abdolamir GhadaksazMohsen Amin

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Methods Mentioned

BETA
fluorescence microscopy
PCR
Flow
FACS
flow cytometry

Software Mentioned

SPSS

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