Anticancer and apoptotic effects on cell proliferation of diosgenin isolated from Costus speciosus (Koen.) Sm

BMC Complementary and Alternative Medicine
Samy Selim, Soad Al Jaouni

Abstract

Diosgenin, a naturally occurring steroid saponin found abundantly in C. speciosus, is a well-known precursor of various synthetic steroidal drugs that are extensively used in the pharmaceutical industry. The present study was conducted to evaluate the in vitro anticancer and apoptotic effects on cell proliferation of diosgenin isolated from C. speciosus (Koen.) Sm. The results indicated that the treatment of HepG2 cells with the sample resulted in a cytotoxic effect as concluded from the IC50 value 32.62 μg/ml, while the treatment of HepG2 cells with paclitaxel, a known anti-cancer drug, resulted in an IC50 value of 0.48 μg/ml. The treatment of MCF-7 cells with the tested sample resulted in high inhibition in the cell viability, and resulted in an IC50 value of 11.03 μg/ml, while the treatment of MCF-7 cells with paclitaxel resulted in an IC50 value of 0.61 μg/ml. The levels of DR4 and caspase-3 were significantly increased (P<0.01) in MCF-7 cells treated with the tested sample compared to untreated cells and possessed a similar activity of paclitaxel in DR4 induction but lower induction in caspase-3. On the other hand the treatment of macrophages or lymphocytes with diosgenin (250 μg/ml) resulted in an induction in the cell pr...Continue Reading

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Citations

May 28, 2019·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Mohammad Reza HajizadehMasoud Torkzadeh-Mahani
Feb 11, 2016·BMC Complementary and Alternative Medicine·Chao ZhangChengwei He
Mar 25, 2020·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Samrin ShaikhVinay Kumar
Jun 29, 2016·The Journal of Membrane Biology·Katarzyna JanickaAneta Dorota Petelska
Jan 25, 2017·Journal of Analytical Methods in Chemistry·Mafalda JesusSamuel Silvestre
Jul 8, 2020·Natural Products and Bioprospecting·Ningbo GongYang Lu
Dec 20, 2020·Biomolecules·Nikita SharmaSurendra Nimesh
Jan 29, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Muhammad Murtala MainasaraYazan K H Ranneh
Apr 11, 2021·Chemistry & Biodiversity·Poonam RawatSharad Srivastava

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Methods Mentioned

BETA
scraping
Assay
flow cytometry
fluorescence microscopy
ELISA
Fluorescence

Software Mentioned

Statistical Package for Social Scientists ( SPSS )

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