Anticancer drug-DNA interactions measured using a photoinduced electron-transfer mechanism based on luminescent quantum dots

Analytical Chemistry
Jipei YuanErkang Wang

Abstract

A sensing system based on the photoinduced electron transfer of quantum dots (QDs) was designed to measure the interaction of anticancer drug and DNA, taking mitoxantrone (MTX) as a model drug. MTX adsorbed on the surface of QDs can quench the photoluminescence (PL) of QDs through the photoinduced electron-transfer process; and then the addition of DNA will bring the restoration of QDs PL intensity, as DNA can bind with MTX and remove it from QDs. Sensitive detection of MTX with the detection limit of 10 nmol L(-1) and a linear detection range from 10 nmol L(-1) to 4.5 micromol L(-1) was achieved. The dependence of PL intensity on DNA amount was successfully utilized to investigate the interactions between MTX and DNA. Both the binding constants and the sizes of binding site of MTX-DNA interactions were calculated based on the equations deduced for the PL recovery process. The binding constant obtained in our experiment was generally consistent with previous reports. The sensitive and speedy detection of MTX as well as the avoidance of modification or immobilization process made this system suitable and promising in the drug-DNA interaction studies.

References

Nov 26, 1998·Biosensors & Bioelectronics·A M BrettS H Serrano
Apr 23, 2003·Journal of Biochemical and Biophysical Methods·Sufen WangCatherine F Yang
Aug 28, 2003·Nature Materials·Igor L MedintzJ Matthew Mauro
Jan 31, 2004·Analytical Chemistry·Ellen R GoldmanHedi Mattoussi
Feb 14, 2004·The Journal of Biological Chemistry·Belinda S ParkerDon R Phillips
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·I L MedintzJ R Deschamps
Aug 13, 2004·Angewandte Chemie·Mark Green
Feb 15, 2005·Journal of Pharmaceutical and Biomedical Analysis·S RaufA M Khalid
Apr 14, 2005·Chemical Reviews·Clemens BurdaMostafa A El-Sayed
May 11, 2005·Biochemical and Biophysical Research Communications·Nan Li, Xiurong Yang
Sep 1, 2005·Journal of the American Chemical Society·Marinella G SandrosDavid E Benson
Dec 22, 2005·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Aaron R ClappHedi Mattoussi
Jul 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ibrahim YildizFrançisco M Raymo
Nov 7, 2006·Biosensors & Bioelectronics·Sakandar RaufAhmad M Khalid
Feb 24, 2007·Journal of the American Chemical Society·Hui PengJadranka Travas-Sejdic
Apr 28, 2007·Physical Chemistry Chemical Physics : PCCP·Françisco M Raymo, Ibrahim Yildiz
Apr 1, 1999·Angewandte Chemie·Shana O KelleyJacqueline K Barton

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Citations

Feb 22, 2013·ACS Applied Materials & Interfaces·Ronit FreemanItamar Willner
Aug 10, 2010·Chemical Communications : Chem Comm·Qinghui ZengHong Zhang
Jan 1, 2009·Sensors·Manuela F Frasco, Nikos Chaniotakis
Mar 25, 2014·Colloids and Surfaces. B, Biointerfaces·Shan HuangBaoqing Hu
Mar 10, 2012·Expert Opinion on Drug Delivery·Mahfoozur RahmanFiroz Anwar
Aug 10, 2010·Advanced Drug Delivery Reviews·Jin XieXiaoyuan Chen
Sep 4, 2012·Luminescence : the Journal of Biological and Chemical Luminescence·Yinping LiJunli Zhang
Jan 27, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Tymish Y OhulchanskyyParas N Prasad
Dec 8, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Nathalie SchleichVéronique Préat
Apr 10, 2012·Drug Discovery Today·Naveed AhmedAbdelhamid Elaissari
Jun 16, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Xuanping TanJidong Yang
Nov 9, 2017·Chemical Communications : Chem Comm·Dan Wen, Alexander Eychmüller
Oct 21, 2010·Analytical and Bioanalytical Chemistry·Shuyan Gao, Naoto Koshizaki
Jan 10, 2012·Analytical and Bioanalytical Chemistry·Zhanguang ChenWenhua Gao
Jan 17, 2020·Journal of Molecular Recognition : JMR·Cheng-Zhang YangYan-Jun Hu
Mar 7, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Shuo HuangMingfeng Wang
Aug 20, 2010·Small·Vidyalakshmi RajendranChristof M Niemeyer
Dec 7, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·W Russ AlgarIgor L Medintz
Jan 12, 2021·The Journal of Physical Chemistry Letters·Hao-Hua DengWei Chen

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