Anticancer effect of bromelain with and without cisplatin or 5-FU on malignant peritoneal mesothelioma cells

Anti-cancer Drugs
Krishna PillaiDavid L Morris

Abstract

Malignant peritoneal mesothelioma (MPM) is a rare neoplasm of the peritoneum, causally related to asbestos exposure. Nonspecific symptoms with a late diagnosis results in poor survival (<1 year). Treatment with cytoreductive surgery and hyperthermic intraperitoneal chemotherapy has improved survival in some patients (median 3-5 years). Hence, new therapies are urgently needed. MUC1 is a glycosylation-dependent protein that confers tumours with invasiveness, metastasis and chemoresistance. Bromelain (cysteine proteinase) hydrolyses glycosidic bonds. Therefore, we investigated the antitumour effect of bromelain on MUC1-expressing MPM cell lines. MUC1 expressions in cells were assessed using immunofluorescent probes with cells grown on cover slips and western blot analysis on cell lysates. The cell lines were treated with various concentrations of bromelain and after 4 and 72 h, their viability was assessed using standard sulforhodamine assays. The cells were also treated with combinations of bromelain and cytotoxic drugs (cisplatin or 5-FU) and their viability was assessed at 72 h. Finally, with western blotting, the effects of bromelain on cellular survival proteins were investigated. PET cells expressed more MUC1 compared with ...Continue Reading

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Citations

Jun 3, 2014·Orphanet Journal of Rare Diseases·Afshin AminiDavid Lawson Morris
Nov 27, 2014·Journal of Experimental & Clinical Cancer Research : CR·Afshin AminiDavid Lawson Morris
Apr 11, 2019·Journal of Medical Signals and Sensors·Farzaneh RaeisiEsfandiar Heidarian
Aug 4, 2020·Journal of Cellular and Molecular Medicine·Shunda WangYupei Zhao
Mar 5, 2016·International Journal of Oncology·Alena MüllerRuben R Plentz
Nov 19, 2019·Journal of Medical Signals and Sensors·Farzane RaeisiYves Lemoigne
May 1, 2021·International Journal of Molecular Sciences·Tomas KoltaiRosa A Cardone
Nov 27, 2021·Integrative Cancer Therapies·Mai H MekkawyOla S Ali

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