Anticancer effect of miR-96 inhibitor in bladder cancer cell lines

Oncology Letters
Ting XuJun Ouyang

Abstract

The present study aimed to investigate the role of microRNA-96 (miR-96) in the proliferation, invasion and apoptosis of bladder cancer cell lines, and the associated mechanisms. The expression of miR-96 and human ether-à-go-go-related (HERG1) potassium channel in the normal uroepithelium SV-HUC-1 cell line, and bladder cancer T24 and 5637 cell lines were examined using reverse transcription-polymerase chain reaction or/and western blotting. Transfection with miR-96 inhibitor or scrambled control (SC) was used to study the biological activities of miR-96 in bladder cancer cell lines. MTT, flow cytometric and Transwell assays were applied to detect cell viability, apoptosis and invasion, respectively. A dual-luciferase reporter assay was applied to determine the association between miR-96 and HERG1 expression. As demonstrated, miR-96 was highly expressed in the two bladder cancer cell lines, particularly in T24 cells. Following transfection with miR-96 inhibitor, miR-96 expression was significantly reduced in the T24 cell line, compared with SC. The miR-96 inhibitor suppressed cell proliferation and invasion, promoted apoptosis and arrested the cell cycle at the G1phase. Consistently, HERG1 was also highly expressed in the two bl...Continue Reading

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Citations

Jan 3, 2019·Cytotechnology·Gizem Ors-KumogluCigir Biray-Avci
Jul 31, 2019·Molecular Neurobiology·Samantha A BanksGarrett A Soukup
Jan 1, 2021·Frontiers in Cell and Developmental Biology·Magdalena BachmannErich Gulbins
Apr 4, 2021·International Journal of Molecular Sciences·Przemysław A StemporPaula Dobosz
Apr 13, 2021·Frontiers in Pharmacology·Verena HofschröerAlbrecht Schwab

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