PMID: 32521910Jun 12, 2020Paper

Anticancer effects of catechin flavonoid in human glioma cells are mediated via autophagy induction, cell cycle arrest, inhibition of cell migration and invasion and targeting MAPK/ERK signalling pathway

Journal of B.U.ON. : Official Journal of the Balkan Union of Oncology
Zhu Zhan Sheng

Abstract

Despite the emergence of innovative cancer treatment strategies, the global burden imposed by malignant glioma is expected to increase. Therefore there is an immediate need to find novel and better approaches for its treatment. The main aim of the current research work was to evaluate the anticancer effects of naturally occurring catechin flavonoid along with examining its effects on cell autophagy, cell cycle phase distribution, cell migration and invasion and MAPK/ERK signalling pathway. MTT cell viability assay was used to assess the effects on cell proliferation and clonogenic assay was used to assess the effects on cell colony formation. Transmission electron microscopy (TEM) and western blot assay were used to examine the effects on autophagy. Flow cytometry was used to assess the effects of catechin on cell cycle, while the effects on cell migration and cell invasion were examined by wound healing assay and transwell chambers assay. Effects on MAPK/ERK signalling pathway were assessed by western blot assay. The results indicated that catechin molecule led to significant and dose-dependent growth inhibitory effects on U87MG human glioma cells with lower cytotoxicity in normal astrocytes. Clonogenic assay indicated strong ...Continue Reading

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