Anticancer effects of miR-200c in colorectal cancer through BMI1

Journal of Cellular Biochemistry
Mitra Karimi MazraehshahRezvan Najafi

Abstract

Colorectal cancer (CRC) is one of the most leading cancer deaths throughout the world. MiR-200c has been shown to have a critical role in cancer initiation and progression. In this study, we investigated the miR-200c expression in CRC tissues and its effects on CRC cell lines which were mediated by polycomb complex protein (BMI1). Quantitative reverse transcription polymerase chain reaction (QRT-PCR) and immunohistochemistry were used to detect miR-200c and BMI1 expression in tumor tissues from 38 patients with CRC and 38 normal colon tissues. HCT-116 and SW-48 cells were transfected by locked nucleic acid (LNA)-anti-miR-200c. Western blot analysis and real-time PCR were applied to determine the BMI1 protein and microRNA (miRNA) levels. The apoptosis was analyzed via annexin/propidium iodide staining, and cell invasion was evaluated by transwell assay. MiR-200c was markedly downregulated in CRC tissues, whereas the protein expression of BMI1 in CRC tissues was upregulated compared with normal colon tissues. In the colon cancer cell lines, transfection of LNA-anti-miR-200c increased BMI1 gene and protein expression as well as the cell invasion. Downregulation of miR-200c by LNA decreased the apoptotic cells. The results from thi...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Oct 28, 2005·The New England Journal of Medicine·Chang-Zheng Chen
Nov 29, 2007·Journal of Surgical Oncology·Jian-Hua LiuKai-Hong Huang
Jul 7, 2009·Acta Biochimica Et Biophysica Sinica·Lili JiangLibing Song
Jan 21, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Rie HamanoYuichiro Doki
Nov 28, 2013·Molecular and Cellular Biology·Xiao Ling LiAshish Lal
Jan 1, 2014·Journal of Cellular Biochemistry·Jianguo CuiJianming Cai
Apr 1, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jianmei ChenYuxiang Chen
Jul 16, 2014·Nature Reviews. Genetics·Jean Hausser, Mihaela Zavolan
Nov 19, 2014·Metabolic Brain Disease·Rezvan NajafiAsieh Hosseini
Dec 17, 2014·The Journal of Biological Chemistry·Manjari DimriGoberdhan P Dimri
Jun 13, 2015·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·M ZuberiA Saxena
Aug 6, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jiwei SunWei Chen
Jul 28, 2016·Journal of Cancer Research and Therapeutics·Raheleh RoudiMitra Mehrazma
Sep 8, 2016·International Journal of Molecular Medicine·Zefeng ZhangWeihong Sha
Jan 24, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Hengchun ZhangHao Jiang
Apr 16, 2017·Pathology Oncology Research : POR·Korosh HeydariRezvan Najafi
Aug 23, 2017·Genetics and Molecular Research : GMR·T ZhangM Deng
Oct 29, 2017·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Fengling LiPeishu Liu
Dec 7, 2017·Experimental Cell Research·Xiu-Feng GongCi-Xiang Zhou
Jan 28, 2016·Signal Transduction and Targeted Therapy·Yong Peng, Carlo M Croce
Jan 9, 2018·Biochemical and Biophysical Research Communications·Li Hongdan, Li Feng

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Citations

Apr 26, 2019·Journal of Cellular Physiology·Saleheh RezapourAli Hassanzadeh
Dec 24, 2018·Diagnostic Pathology·Mohammad Reza Abbaszadegan, Meysam Moghbeli
Oct 22, 2020·Biotechnology and Applied Biochemistry·Mona PourjafarRezvan Najafi
Oct 21, 2020·European Journal of Gastroenterology & Hepatology·Noha G Bader El DinAhmed Khairy
Feb 12, 2020·Microbial Pathogenesis·Rasoul MirzaeiRasoul Yousefimashouf
Apr 4, 2021·International Journal of Molecular Sciences·Przemysław A StemporPaula Dobosz
Aug 3, 2021·BioMed Research International·Mahsa RezaeepoorRezvan Najafi

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