PMID: 19148534Jan 17, 2009Paper

Anticancer effects on TACC3 by treatment of paclitaxel in HPV-18 positive cervical carcinoma cells

Oncology Reports
Eun-Kyoung YimJong-Sup Park

Abstract

Previously, we used proteome analysis to identify transforming acidic coiled coil (TACC) 3 as a protein that is down-regulated upon paclitaxel treatment in cervical cancer cells. TACC3 mRNA and protein levels decreased after paclitaxel treatment in a time- and dose-dependent manner, and the transactivation of TACC3 promoter was dramatically diminished by paclitaxel. Importantly, paclitaxel treatment and knockdown of TACC3 by siRNA led to a synergistic enhancement of significant G2/M phase arrest and apoptosis in HeLa cells. In contrast to TACC3-deficient cells, paclitaxel treatment of mTACC3-overexpressing cells failed to induce G2/M phase arrest, cell growth inhibition, and apoptotic cell death. We studied the associated gene in mTACC overexpressed cells using microarray. From these results, numerous genes have been identified as being associated with tumor progression (Ppia, TMSB10, Annexin A2, rab31, prostaglandin E2-EP2, UHRF1), chemoresistance (Akt, Plk-1, MAP kinase) and metastasis (MMP9, PECAM-1) in mTACC3 overexpressed HeLa cells. Thus, TACC3 is thought to be the critical molecule in mediating the anticancer mechanisms of paclitaxel in p53 inactivated cells by inducing G2/M arrest and apoptosis. And our data suggested t...Continue Reading

Citations

May 25, 2010·Archives of Gynecology and Obstetrics·Ling-Min LiaoHui-Yun Wang
Apr 30, 2013·Cancer Letters·Geun-Hyoung HaEun-Kyoung Yim Breuer
Aug 8, 2012·Molecular and Cellular Biology·Kathryn M CappellAngelique W Whitehurst
Oct 11, 2011·BMC Genomics·Florbela A VieiraDeborah M Power
Aug 28, 2012·Molecular Cancer·Bettina GrismayerViktor Magdolen
Aug 13, 2013·PloS One·Geun-Hyoung HaEun-Kyoung Yim Breuer
Dec 5, 2014·Journal of Cellular and Molecular Medicine·Christelle En Lin Chua, Bor Luen Tang
Oct 5, 2014·Cancer Letters·Rehana QureshiM A Rizvi

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