Anticancer potential against cervix cancer (HeLa) cell line of probiotic Lactobacillus casei and Lactobacillus paracasei strains isolated from human breast milk

Food & Function
Muhammad Shahid Riaz RajokaJunling Shi

Abstract

Lactic acid bacteria have been categorized as probiotics and play a crucial role in human health by stimulating the supply of nutrients, shaping the immune system, and preventing the colonization of pathogenic microbes. This study investigated the mechanisms for the action of three potential probiotic Lactobacillus strains: Lactobacillus casei SR1, Lactobacillus casei SR2, and Lactobacillus paracasei SR4 isolated from human breast milk. These Lactobacillus strains were identified via 16S DNA sequencing and characterized via biochemical assays including acid resistance, bile resistance, antioxidant activity, and antibiotic susceptibility. The bioactivity of the cell-free culture supernatant (CFCS) secreted by these strains on the cervix cancer (HeLa) cell line was also evaluated via cytotoxicity assay and apoptosis analysis. The mechanism of anticancer activity was also investigated via RT-qPCR and western blotting. The results demonstrated that these newly isolated Lactobacillus strains from human milk displayed noticeable probiotic characteristics such as excellent antibiotic susceptibility, outstanding antioxidant activity, and promising resistance to low pH and high concentration of bile salts. The results of the conducted b...Continue Reading

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Citations

Jun 5, 2019·BMC Complementary and Alternative Medicine·Li-Oon ChuahKhatijah Yusoff
Jun 13, 2020·Journal of Ovarian Research·Moghaddaseh JahanshahiShahla Chaichian
Nov 8, 2018·BioMed Research International·Dorota Zielińska, Danuta Kolożyn-Krajewska
Feb 19, 2021·Biotechnology Reports·Tayebeh Jafari-NasabSadegh Khorrami
Jul 10, 2019·Food Research International·Muhammad Shahid Riaz RajokaJunling Shi
Mar 23, 2021·Advanced Pharmaceutical Bulletin·Aziz Homayouni RadAmin Abbasi
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chau Ha PhamHee Min Yoo
Oct 3, 2021·Probiotics and Antimicrobial Proteins·Shiyue HuHongpeng He

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Methods Mentioned

BETA
flow cytometry
PCR
fluorescence microscopy
protein assay

Software Mentioned

ClustalW2
Mega
Basic Local Alignment Tool ( BLAST )

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