Anticancer properties of Semen Euphorbiae towards ACHN human renal adenocarcinoma cells by inducing apoptosis

Saudi Journal of Biological Sciences
Hua-Wen ChengWen-Yan He

Abstract

The main objective of the present research work was to evaluate the antitumor effects of ethanol extract of Semen Euphorbiae (EESE) in ACHN human renal carcinoma cells. The effects on apoptosis induction, cell cycle phase distribution and livin protein expression were also evaluated. MTT assay was used to assess cytotoxic effects of the extract while as clonogenic assay was used to evaluate effects on colony formation tendency. Inverted phase contrast and fluorescence microscopic techniques were used to evaluate effects of EESE on cellular morphology and apoptosis. Flow cytometry using annexin V-FITC and propidium iodide were used to quantify the extent of apoptosis and also to evaluate effects on cell cycle. Results indicate that ethanol extract of Semen Euphorbiae exhibited potent cytotoxic effects in ACHN human renal cancer cells. These effects were found to be dose-dependent as well as time dependent. Clonogenic assay revealed that EESE led to dose-dependent inhibition of colony formation in these cells. EESE-treated cells also showed evident signs of alterations and deformations in cell morphology including detachment of cells from one another forming small cluster of cells. In contrast to untreated control cells, EESE-tre...Continue Reading

Citations

Aug 28, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Hui-Nan WangYing-Zi Wang

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Methods Mentioned

BETA
surgical resection
ELISA
fluorescence microscopy
flow cytometry

Software Mentioned

Cell Quest

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