Anticancer siRNA cocktails as a novel tool to treat cancer cells. Part (A). Mechanisms of interaction

International Journal of Pharmaceutics
Maksim IonovMaria Bryszewska

Abstract

This paper examines a perspective on the use of newly engineered nanomaterials as effective and safe carriers of genes for the therapy of cancer. Three different groups of cationic dendrimers (PAMAM, phosphorus and carbosilane) were complexed with anticancer siRNA and their biophysical properties of the dendriplexes analyzed. The potential of the dendrimers as nanocarriers for anticancer siBcl-xl, siBcl-2, siMcl-1 siRNAs and a siScrambled sequence was explored. Dendrimer/siRNA complexes were characterized by methods including fluorescence, zeta potential, dynamic light scattering, circular dichroism, gel electrophoresis and transmission electron microscopy. Some of the experiments were done with heparin to check if siRNA can be easily disassociated from the complexes, and whether released siRNA maintains its structure after interaction with the dendrimer. The results indicate that siRNAs form complexes with all the dendrimers tested. Oligoribonucleotide duplexes can be released from dendriplexes after heparin treatment and the structure of siRNA is maintained in the case of PAMAM or carbosilane dendrimers. The dendrimers were also effective in protecting siRNA from RNase A activity. The selection of the best siRNA carrier will ...Continue Reading

References

May 19, 1997·Cancer Letters·H SuzukiH Ishii
Jan 29, 2000·Biotechnology and Bioengineering·D V SchafferD A Lauffenburger
Jun 21, 2001·Journal of the American Chemical Society·D L BogerM P Hedrick
Jan 29, 2003·International Journal of Pharmaceutics·R JevprasesphantA D'Emanuele
Nov 5, 2003·Journal of Cellular and Molecular Medicine·Alexandrina Burlacu
Dec 6, 2003·Pharmacological Reviews·Ollivier MilhavetMark P Mattson
May 24, 2005·Nature Biotechnology·Erwei SongJudy Lieberman
Mar 14, 2008·Bioconjugate Chemistry·Hirohito AyameKazunari Akiyoshi
Jul 5, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Miriam BreunigAchim Goepferich
Aug 30, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Nick WeberM Angeles Muñoz-Fernández
Nov 4, 2008·Nature Medicine·Hendrik PoeckGunther Hartmann
Feb 5, 2009·Nucleic Acids Research·Jaroslav KyprMichaela Vorlícková
Feb 6, 2009·Biotechnology Progress·Maggie LawP Chen
Sep 23, 2009·International Journal of Pharmaceutics·Antos ShakhbazauMaria Bryszewska
Oct 8, 2009·Dalton Transactions : an International Journal of Inorganic Chemistry·Beatriz RasinesF Javier de la Mata
Apr 23, 2010·RNA·Katrin TiemannPål Saetrom
Jun 15, 2010·The AAPS Journal·Jie WangJessie L-S Au
Dec 2, 2010·Organic & Biomolecular Chemistry·Julia Morales-SanfrutosFrancisco Santoyo-Gonzalez
Mar 24, 2011·Bioconjugate Chemistry·Douglas G MullenMark M Banaszak Holl
Dec 17, 2011·Chemistry and Physics of Lipids·Maksim IonovMaria Bryszewska
May 12, 2012·Biochimica Et Biophysica Acta·Maksim IonovMaria Bryszewska
Sep 27, 2012·Colloids and Surfaces. B, Biointerfaces·Maksim IonovMaria Bryszewska
Dec 29, 2012·Cellular & Molecular Biology Letters·Hitesh JaganiSagar Gang
Mar 20, 2013·Nanomedicine : Nanotechnology, Biology, and Medicine·E Vacas CórdobaM A Muñoz-Fernández
Mar 29, 2013·International Journal of Pharmaceutics·Zarrintaj ZiraksazMajid Tabbakhian
Oct 4, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Henryk DudekBob D Brown
Mar 13, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Dzmitry ShcharbinMaria Bryszewska

❮ Previous
Next ❯

Citations

Jan 3, 2016·International Journal of Pharmaceutics·Maksim IonovMaria Bryszewska
Jan 22, 2017·Trends in Biotechnology·Joanna K L WongAlexander M Seifalian
Mar 10, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anne-Marie Caminade, Jean-Pierre Majoral
Aug 23, 2017·Organic & Biomolecular Chemistry·Carlos E Gutierrez-UlloaRafael Gómez
Apr 23, 2019·Journal of Biomedical Materials Research. Part a·Anjali JainMaksim Ionov
May 6, 2020·International Journal of Molecular Sciences·Michał GorzkiewiczBarbara Klajnert-Maculewicz
Jul 8, 2020·International Journal of Molecular Sciences·Elżbieta Pędziwiatr-WerbickaMaria Bryszewska
Aug 12, 2018·Pharmaceutics·Volha DzmitrukMaria Bryszewska
Jan 12, 2019·Pharmaceutical Development and Technology·Upendra BulbakeWahid Khan
Jul 10, 2019·Future Medicinal Chemistry·Sylwia MichlewskaMaria Bryszewska
Aug 10, 2017·Applied Microbiology and Biotechnology·Chit TamTzi Bun Ng
Jan 23, 2021·The Journal of Physical Chemistry. B·Elżbieta Pędziwiatr-WerbickaMaria Bryszewska

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Malgorzata FerencMaria Bryszewska
Colloids and Surfaces. B, Biointerfaces
Dzmitry ShcharbinMaria Bryszewska
© 2021 Meta ULC. All rights reserved