PMID: 20650103Aug 1, 1995Paper

Anticonvulsant drug toxicity in rat brain cell aggregate cultures

Toxicology in Vitro : an International Journal Published in Association with BIBRA
B SchilterP Honegger

Abstract

As an extension of a previous validation study, the concentration-dependent effects of a series of anticonvulsant drugs were examined in aggregating cell cultures of foetal rat telencephalon. Cultures were treated either at an early (day 5 to day 14) or at an advanced (day 20 to day 28) developmental stage, and assayed for changes in the activities of the cell type-specific enzymes choline acetyltransferase (ChAT), acetylcholinesterase (AChE), glutamic acid decarboxylase (GAD), glutamine synthetase (GS) and 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNP). Five drugs (carbamazepine, diazepam, phenobarbital, phenytoin and valproate), currently used in the treatment of epileptic patients, were tested together with losigamone, a recently developed anticonvulsant. The results show distinct, concentration-dependent patterns of biochemical changes for the different drugs. Phenytoin, carbamazepine, losigamone and diazepam greatly reduced GAD, ChAT and AChE activities, indicating a relatively high neuron-specific toxic potential. Diazepam produced a more general pattern of toxicity and, in contrast to the anticonvulsants, showed higher toxicity in less-differentiated cultures. Phenobarbital and valproate slightly but significantly inc...Continue Reading

References

Apr 1, 1979·Analytical Biochemistry·M R Pishak, A T Phillips
May 1, 1990·Developmental Medicine and Child Neurology·L LaegreidL Abrahamsson
Jan 1, 1990·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·C M ReganD C Williams
May 1, 1989·Neurotoxicology and Teratology·C V Vorhees, D R Minck
Jun 1, 1987·Teratology·E J LammerG P Oakley
Oct 15, 1988·Experientia·P Honegger, P Werffeli
Jun 1, 1986·Teratology·J W Hanson
Jul 1, 1966·Analytical Biochemistry·D J ReedC H Wang
Oct 1, 1967·Journal of Neurochemistry·B K Schrier, L Shuster
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·E MatthewD L Engelhardt
Nov 1, 1984·American Journal of Medical Genetics·T E Kelly
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J K WangS Spector
Jul 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·F Monnet-TschudiP Honegger
Nov 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·P KuceraB Schmid

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Citations

Dec 19, 2012·Psychiatry Research·Roger B VarelaJoão Quevedo
Apr 29, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Bhavesh K Ahir, Margaret K Pratten
Aug 15, 2002·CNS Drug Reviews·Robert Hoerr, Michael Noeldner

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