Abstract
Besides being present in paraneoplastic pemphigus (PNP), circulating antidesmoplakin (DP) antibodies have been found anecdotally in other bullous diseases, including pemphigus foliaceus and pemphigus vulgaris. To verify how frequent anti-DP antibodies are in pemphigus vulgaris. We studied 48 sera from patients with proven pemphigus vulgaris (29 mucosal dominant pemphigus and 19 mucocutaneous pemphigus) by indirect immunofluorescence (IIF) with rat bladder epithelium (RBE) as a substrate and by immunoblotting (IB) on human keratinocyte cultures enriched in DP. Ten sera (21%) were positive in IIF on RBE. By IB, eight sera proved to have antibodies to both DP I (250 kDa) and DP II (210 kDa), one serum had antibodies directed to DP I only, and two sera to DP II only. Our data confirm that RBE is not a specific IIF substrate for the serological diagnosis of PNP. It remains a sensitive and specific substrate for the detection of anti-DP antibodies, which, in patients with pemphigus vulgaris, are probably caused by an epitope-spreading phenomenon.
References
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jun 1, 1992·International Journal of Biological Macromolecules·K J GreenD A Parry
Jun 1, 1992·The Journal of Clinical Investigation·J R OurslerG J Anhalt
Mar 1, 1991·Experimental Cell Research·K J GreenL A Nilles
Dec 20, 1990·The New England Journal of Medicine·G J AnhaltL Ariss-Abdo
Aug 15, 1970·Nature·U K Laemmli
May 1, 1993·Journal of the American Academy of Dermatology·A Y LiuW F Bergfeld
May 1, 1995·The Journal of Investigative Dermatology·T HashimotoS Gamou
Mar 1, 1995·Journal of the American Academy of Dermatology·J HelouG J Anhalt
Nov 1, 1994·The British Journal of Dermatology·T HashimotoT Nishikawa
Jul 1, 1994·The Journal of Investigative Dermatology·P JolyF Tron
Jun 1, 1993·Journal of Cutaneous Pathology·D R MehreganM S Peters
May 1, 1996·The Journal of Investigative Dermatology·D FoedingerK Rappersberger
May 1, 1997·The British Journal of Dermatology·M OkuraM Mizoguchi
Sep 1, 1997·The Journal of Investigative Dermatology·S C KimT G Lee
Aug 12, 1998·The Journal of Investigative Dermatology·M G MahoneyJ R Stanley
Aug 26, 1998·The Journal of Clinical Investigation·M AmagaiT Hashimoto
Oct 23, 1998·Journal of the European Academy of Dermatology and Venereology : JEADV·D Jiao, J C Bystryn
May 3, 2000·The British Journal of Dermatology·P MartelP Joly
Sep 27, 2000·Journal of the American Academy of Dermatology·P JolyF Tron
Dec 12, 2001·The British Journal of Dermatology·S C KimM Amagai
Jul 10, 2004·Journal of the American Academy of Dermatology·Daniel MimouniCarlos H Nousari
Citations
Apr 21, 2007·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·C Mühlhoff, M Megahed
Nov 9, 2011·Nature Reviews. Rheumatology·Darren D O'Rielly, Proton Rahman
Nov 26, 2015·Seminars in Immunopathology·Giovanni Di ZenzoLuca Borradori
Sep 10, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Christian ProbstLars Komorowski
Nov 4, 2008·Veterinary Immunology and Immunopathology·Atsushi YabuzoeToshiroh Iwasaki
Oct 10, 2006·Tissue Antigens·G Tchernev, C E Orfanos
Oct 24, 2008·The British Journal of Dermatology·A B Alexandroff, K E Harman
Jul 5, 2012·The Australasian Journal of Dermatology·Angeline Anning Yong, Hong Liang Tey
Jun 26, 2013·The British Journal of Dermatology·A M PootH H Pas
Nov 6, 2009·The British Journal of Dermatology·S Grootenboer-MignotS Chollet-Martin
Jun 26, 2015·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Enno SchmidtAlexander Nast
Nov 12, 2015·The British Journal of Dermatology·A M PootG F H Diercks
Dec 1, 2017·International Journal of Molecular Sciences·Giovanni PaolinoStefano Calvieri
Jan 27, 2018·International Journal of Molecular Sciences·Quincy A Quick
Feb 26, 2010·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Jutta ZimmermannEnno Schmidt
Dec 27, 2018·The British Journal of Dermatology·S SunX Zeng
May 3, 2018·Frontiers in Immunology·Dario Didona, Giovanni Di Zenzo