Antidiabetic drug metformin suppresses endotoxin-induced uveitis in rats.

Investigative Ophthalmology & Visual Science
Nilesh M KalariyaKota V Ramana

Abstract

To investigate the therapeutic effects of metformin, a commonly used antidiabetic drug, in preventing endotoxin-induced uveitis (EIU) in rats. EIU in Lewis rats was developed by subcutaneous injection of lipopolysaccharide (LPS; 150 μg). Metformin (300 mg/kg body weight, intraperitoneally) or its carrier was injected either 12 hours before or 2 hours after LPS induction. Three and 24 hours after EIU, eyes were enucleated and aqueous humor (AqH) was collected. The MILLIPLEX-MAG Rat cytokine-chemokine magnetic bead array was used to determine inflammatory cytokines. The expression of Cox-2, phosphorylation of AMPK, and NF-κB (p65) were determined immunohistochemically. Primary human nonpigmented ciliary epithelial cells (HNPECs) were used to determine the in vitro efficacy of metformin. Compared with controls, the EIU rat AqH had significantly increased number of infiltrating cells and increased levels of various cytokines and chemokines (TNF-α, MCP-1, IL-1β, MIP-1α, IL-6, Leptin, and IL-18) and metformin significantly prevented the increase. Metformin also prevented the expression of Cox-2 and phosphorylation of p65, and increased the activation of AMPK in the ciliary bodies and retinal tissues. Moreover, metformin prevented the...Continue Reading

Citations

Jan 8, 2014·Nature Reviews. Endocrinology·Ida Pernicova, Márta Korbonits
Feb 15, 2014·Current Diabetes Reports·Anusha Jayaraman, Christian J Pike
Oct 9, 2014·Journal of Diabetes Research·Yusuke KemmochiAkihiro Kakehashi
Dec 8, 2015·Expert Opinion on Investigational Drugs·Raquel Salazar-MéndezMiguel Cordero-Coma
Nov 28, 2012·European Journal of Internal Medicine·Khalid MahmoodNasir Ali Rahimnajjad
Dec 23, 2015·Assay and Drug Development Technologies·Hermann A M Mucke
Apr 23, 2015·Biochemical and Biophysical Research Communications·Simona StavriAnca V Gafencu
Sep 14, 2016·International Journal of Pediatric Otorhinolaryngology·Jae Gu ChoSung Won Chae
Aug 12, 2016·Drug Development Research·Abhimanu Pandey, Vijay L Kumar
Mar 23, 2018·The British Journal of Ophthalmology·Takayo WatanabeAnnabelle A Okada
Aug 23, 2020·Indian Journal of Ophthalmology·Mehmet BalbabaYesari Eröksüz
Jan 10, 2017·Human Molecular Genetics·Dimitra AthanasiouMichael E Cheetham
Oct 14, 2020·Naunyn-Schmiedeberg's Archives of Pharmacology·Nazmun NaharNorshahira Solehah Umran
May 8, 2020·Seminars in Cancer Biology·Anuradha KirtoniaManoj Garg

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