Antifibrinolytic role of a bee venom serine protease inhibitor that acts as a plasmin inhibitor.

PloS One
Young Moo ChooByung Rae Jin

Abstract

Bee venom is a rich source of pharmacologically active substances. In this study, we identified a bumblebee (Bombus ignitus) venom Kunitz-type serine protease inhibitor (Bi-KTI) that acts as a plasmin inhibitor. Bi-KTI showed no detectable inhibitory effect on factor Xa, thrombin, or tissue plasminogen activator. In contrast, Bi-KTI strongly inhibited plasmin, indicating that it acts as an antifibrinolytic agent; however, this inhibitory ability was two-fold weaker than that of aprotinin. The fibrin(ogen)olytic activities of B. ignitus venom serine protease (Bi-VSP) and plasmin in the presence of Bi-KTI indicate that Bi-KTI targets plasmin more specifically than Bi-VSP. These findings demonstrate a novel mechanism by which bumblebee venom affects the hemostatic system through the antifibrinolytic activity of Bi-KTI and through Bi-VSP-mediated fibrin(ogen)olytic activities, raising interest in Bi-KTI and Bi-VSP as potential clinical agents.

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Citations

Mar 19, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Hu WanByung Rae Jin
Jul 21, 2012·Cutaneous and Ocular Toxicology·Sang Mi HanSok Cheon Pak
Oct 26, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Hu WanByung Rae Jin
Oct 1, 2015·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Jeilyn VivasBelsy Guerrero
Sep 13, 2015·Toxicon : Official Journal of the International Society on Toxinology·Li DingZongyun Chen
Mar 25, 2014·Medicinal Research Reviews·Rami A Al-Horani, Umesh R Desai
Jun 12, 2013·Toxicon : Official Journal of the International Society on Toxinology·Zongyun ChenYingliang Wu
Jun 14, 2015·Toxicon : Official Journal of the International Society on Toxinology·Matthias Van VaerenberghDirk C de Graaf
Jun 19, 2013·Marine Drugs·Caroline B F Mourão, Elisabeth F Schwartz
Nov 16, 2017·Toxins·Nezahat Pınar BarkanDuygu Ozel Demiralp
Mar 7, 2019·Biochemical Society Transactions·Guojie WuRuby H P Law
Feb 23, 2021·Homeopathy : the Journal of the Faculty of Homeopathy·Silvia Waisse
Apr 11, 2021·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Bo Yeon KimByung Rae Jin
May 22, 2016·Developmental and Comparative Immunology·Kwang Sik LeeByung Rae Jin
May 25, 2021·International Journal of Biological Macromolecules·Zelinda Schemczssen-GraeffSilvio Sanches Veiga
May 18, 2021·Pesticide Biochemistry and Physiology·Caihong ZhangGemei Liang
Oct 29, 2019·Journal of Medicinal Chemistry·Torsten SteinmetzerSimon J A Wiedemeyer
Dec 30, 2017·Journal of Proteome Research·Qiwen LiaoSimon Ming-Yuen Lee
Jan 7, 2022·Cellular and Molecular Life Sciences : CMLS·Zhiyi LiaoRen Lai

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Datasets Mentioned

BETA
JN381496

Methods Mentioned

BETA
electrophoresis
electrophoretic mobility shift assay
Protein Purification
Protein Assay
X-ray

Software Mentioned

BLAST
DNASIS

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