Mar 5, 2009

Antifibrotic effect of edaravone in rat liver cirrhosis induced by dimethylnitrosamine

Clinical and Experimental Medicine
Hiroto TanakaMasakazu Ichinose

Abstract

Edaravone (EDA), a newly synthesized free radical scavenger, has shown excellent results in the treatment of stroke. An overproduction of reactive oxygen species (ROS) causing oxidative DNA damage has been accounted as a major factor causing liver injury and fibrosis. Therefore, we examined its effect of EDA in rat model of liver cirrhosis induced by dimethylnitrosamine (DMN). Ten rats (DMN-group) were injected intraperitoneally with DMN (10 microg/g body weight) alone and another ten rats (EDA-group) were injected intraperitoneally with EDA (10 mg/kg body weight) 2 h after being injected with DMN. Both groups underwent their injection regimen three times a week for 4 weeks, after which the rats were sacrificed and their liver tissue sections were stained with Azan-Mallory for quantitative analyses of fibrosis development, using soft imaging and a previously published scoring system. Additionally, these sections were immunohistochemically stained using an antibody against alpha-smooth muscle actin (alpha-SMA). The total-bililubin in the EDA-group was found to be lower than that in the DMN-group. Quantitive analysis of liver fibrosis showed that the fibrotic area of the EDA-group was significantly smaller than that of the DMN-gr...Continue Reading

  • References27
  • Citations2

References

  • References27
  • Citations2

Citations

Mentioned in this Paper

Phenylmethylpyrazolone
Cirrhosis
Free Radicals
MCI 186
Weighing Patient
Bilirubin, (4E,15E)-Isomer
Severity of Illness Index
Fibrosis, Liver
Pyramidone
Visual Suppression

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