Antifibrotic properties of caveolin-1 scaffolding domain in vitro and in vivo.

American Journal of Physiology. Lung Cellular and Molecular Physiology
Elena TourkinaStanley Hoffman

Abstract

Lung fibrosis involves the overexpression of ECM proteins, primarily collagen, by alpha-smooth muscle actin (ASMA)-positive cells. Caveolin-1 is a master regulator of collagen expression by cultured lung fibroblasts and of lung fibrosis in vivo. A peptide equivalent to the caveolin-1 scaffolding domain (CSD peptide) inhibits collagen and tenascin-C expression by normal lung fibroblasts (NLF) and fibroblasts from the fibrotic lungs of scleroderma patients (SLF). CSD peptide inhibits ASMA expression in SLF but not NLF. Similar inhibition of collagen, tenascin-C, and ASMA expression was also observed when caveolin-1 expression was upregulated using adenovirus. These observations suggest that the low caveolin-1 levels in SLF cause their overexpression of collagen, tenascin-C, and ASMA. In mechanistic studies, MEK, ERK, JNK, and Akt were hyperactivated in SLF, and CSD peptide inhibited their activation and altered their subcellular localization. These studies and experiments using kinase inhibitors suggest many differences between NLF and SLF in signaling cascades. To validate these data, we determined that the alterations in signaling molecule activation observed in SLF also occur in fibrotic lung tissue from scleroderma patients a...Continue Reading

References

Nov 15, 1975·Experientia·N ReverdinY Kapanci
Jan 1, 1995·International Reviews of Immunology·J Van de WaterM E Gershwin
Jun 1, 1995·American Journal of Respiratory Cell and Molecular Biology·A J GrayG J Laurent
Jan 1, 1995·Molecular Membrane Biology·M P LisantiM Sargiacomo
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·F GalbiatiM P Lisanti
Oct 6, 1998·Annual Review of Biochemistry·R G Anderson
Nov 14, 1998·The American Journal of Physiology·P W Shaul, R G Anderson
Mar 20, 1999·The Journal of Biological Chemistry·C V CarmanJ L Benovic
Apr 14, 1999·The Journal of Investigative Dermatology·T YamamotoK Nishioka
Mar 18, 2000·The Journal of Biological Chemistry·A Toker, A C Newton
Jul 18, 2001·The Journal of Biological Chemistry·B RazaniM P Lisanti
Nov 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·G SowaW C Sessa
Aug 22, 2002·The Journal of Biological Chemistry·Pingsheng LiuRichard G W Anderson
Sep 12, 2002·Pharmacological Reviews·Babak RazaniMichael P Lisanti
Oct 22, 2002·American Journal of Physiology. Cell Physiology·Alex W CohenMichael P Lisanti
Oct 22, 2002·American Journal of Physiology. Renal Physiology·Hong HuaCatharine I Whiteside
Apr 18, 2003·American Journal of Physiology. Cell Physiology·Satoshi NumazawaTakemi Yoshida
Jan 10, 2004·The Journal of Urology·Scott E WoodmanMichael P Lisanti
Jan 27, 2004·American Journal of Respiratory Cell and Molecular Biology·Elena TourkinaStanley Hoffman
Feb 24, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Xiaopeng LiBruce D Uhal
Sep 28, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Galina S BogatkevichAnna Ludwicka-Bradley
Jan 8, 2005·The Journal of Biological Chemistry·Jian CuiAlbert J Wong
Jan 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·Pascal N BernatchezWilliam C Sessa
Feb 26, 2005·Circulation Research·Daniel G SeddingRuediger C Braun-Dullaeus
Mar 4, 2005·The American Journal of Pathology·Bethany B MooreGalen B Toews
Apr 28, 2005·The Journal of Investigative Dermatology·Yoshihiro MimuraKunihiko Tamaki
May 28, 2005·The American Journal of Pathology·Mehrnaz Gharaee-KermaniSem H Phan
Sep 24, 2005·American Journal of Respiratory and Critical Care Medicine·Eric S WhiteGalen B Toews
Dec 17, 2005·American Journal of Respiratory Cell and Molecular Biology·Xiao Mei WangAugustine M K Choi
Mar 30, 2006·Current Rheumatology Reports·Timothy E QuanRichard Bucala
Jun 23, 2006·The New England Journal of Medicine·Donald P TashkinUNKNOWN Scleroderma Lung Study Research Group

❮ Previous
Next ❯

Citations

May 1, 2012·British Journal of Anaesthesia·K J GrimC M Pabelick
Sep 17, 2008·Rheumatology·R M Silver, A U Wells
Oct 28, 2008·Current Opinion in Rheumatology·Francesco Del GaldoSergio A Jimenez
Sep 8, 2012·Current Opinion in Rheumatology·Richard M SilverStanley Hoffman
Dec 17, 2008·Current Opinion in Nephrology and Hypertension·Paul A Insel, Hemal H Patel
Oct 2, 2008·Acta Physiologica·A Rahman, K Swärd
Apr 23, 2010·Annals of the Rheumatic Diseases·Elena TourkinaStanley Hoffman
Dec 5, 2008·American Journal of Physiology. Cell Physiology·Premanand C SundivakkamChinnaswamy Tiruppathi
Dec 6, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yashodhar P BhandarySreerama Shetty
Oct 14, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·O Le SauxC Jourdan-Le Saux
May 13, 2011·American Journal of Respiratory Cell and Molecular Biology·Steffen KunzmannBoris W Kramer
Mar 1, 2012·American Journal of Respiratory Cell and Molecular Biology·Pooja ShivshankarClaude Jourdan Le Saux
Jan 14, 2012·The European Respiratory Journal·Venkatachalem SathishChristina M Pabelick
Sep 10, 2013·Respiratory Research·Charles ReeseElena Tourkina
Sep 14, 2011·International Journal of Rheumatology·Stéphane Chabaud, Véronique J Moulin
Apr 2, 2009·Nature Reviews. Rheumatology·John Varga, Boris Pasche
Jan 26, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Sergio A Jimenez, Sonsoles Piera-Velazquez
Jan 19, 2016·Trends in Molecular Medicine·Eva A Rog-ZielinskaRoger Markwald
Jul 3, 2015·The Journal of Investigative Dermatology·Yukie YamaguchiMichiko Aihara
Aug 10, 2010·Clinics in Chest Medicine·Faye N HantRichard M Silver
Mar 8, 2011·The British Journal of Dermatology·G-Y ZhangW-Y Gao
Mar 30, 2010·Journal of Cellular and Molecular Medicine·Yukie YamaguchiCarol A Feghali-Bostwick
Jan 27, 2012·Journal of Cellular and Molecular Medicine·Paul HainesAndreea M Bujor
Sep 11, 2010·Current Opinion in Immunology·Adrian R Kendal, Herman Waldmann
Apr 9, 2015·Clinical and Translational Medicine·Peter J Wermuth, Sergio A Jimenez
Sep 27, 2013·Cell Cycle·Cecilia J G de AlmeidaMichael P Lisanti
May 20, 2014·Free Radical Biology & Medicine·Feng ChenDavid J R Fulton
Apr 16, 2013·Matrix Biology : Journal of the International Society for Matrix Biology·David GvaramiaVincent Everts
Sep 10, 2014·Frontiers in Pharmacology·Eleonora Cianci, Antonio Recchiuti
Jul 8, 2014·Frontiers in Pharmacology·Charles ReeseStanley Hoffman
Dec 6, 2014·Current Rheumatology Reports·Sonsoles Piera-Velazquez, Sergio A Jimenez

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