Abstract
Efficacious therapeutic strategies against lymphatic filariasis are always sought after. However, natural products are a promising resource for developing effective antifilarial agents. Azadirachtin, a significant tetranortriterpenoid phytocompound found in Azadirachta indica, was evaluated in vitro for antifilarial potential against the filarial parasite Setaria cervi. Dye exclusion and MTT assay confirmed the antifilarial potential of azadirachtin against S. cervi with a median lethal dose (LC50) of 6.28 μg/ml for microfilariae (mf), and 9.55 μg/ml for adult parasites. Morphological aberrations were prominent in the histological sections of the azadirachtin-exposed parasites. Moreover, alterations in the reactive oxygen species (ROS) parameters in treated parasites were evident. Induction of apoptosis in treated parasites was confirmed by DNA laddering, acridine orange (AO)/ethidium bromide (EtBr) double staining and in situ DNA fragmentation. The downregulation of anti-apoptotic CED-9 and upregulation of proapoptotic EGL-1, CED-4 and CED-3 at both the transcription and translation levels confirmed apoptosis execution at the molecular level. Changes in the gene expressions of nuc-1, cps-6 and crn-1 further clarified the molec...Continue Reading
References
May 7, 1976·Analytical Biochemistry·M M Bradford
Apr 1, 1987·Immunological Investigations·N A KaushalS Ghatak
Mar 3, 1997·FEBS Letters·A GormanT G Cotter
Mar 21, 2001·Apoptosis : an International Journal on Programmed Cell Death·H U SimonF Levi-Schaffer
Apr 21, 2001·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·R B RaizadaR P Singh
Dec 31, 2002·The Journal of Eukaryotic Microbiology·Oliver BillkerRobert E Sinden
Jul 4, 2003·The EMBO Journal·Jay Z ParrishDing Xue
Jun 9, 2004·Chembiochem : a European Journal of Chemical Biology·Goutam Brahmachari
Jul 21, 2004·Journal of Ethnopharmacology·Sara J BoekeIvonne M C M Rietjens
Dec 1, 1992·Parasitology Today·P M Brophy, D I Pritchard
Jan 25, 2005·Fitoterapia·Vandna MishraNizam U Khan
Feb 24, 2006·Nature Reviews. Molecular Cell Biology·Guillaume Lettre, Michael O Hengartner
Nov 9, 2007·PLoS Neglected Tropical Diseases·Sara Lustigman, James P McCarter
Oct 3, 2008·The Korean Journal of Parasitology·R K Shenoy
Jan 5, 2010·Free Radical Biology & Medicine·Magdalena L Circu, Tak Yee Aw
Jan 1, 2008·CSH Protocols·Andrew H FischerRolf Zeller
Nov 18, 2011·Journal of Assisted Reproduction and Genetics·Anima TripathiShail K Chaube
May 9, 2012·Parasitology International·Prasanta SainiSanti P Sinha Babu
May 9, 2012·Parasitology Research·Ananya NayakSanti P Sinha Babu
Nov 28, 2013·Experimental Parasitology·Niladri MukherjeeSanti P Sinha Babu
Jun 4, 2014·Cold Spring Harbor Protocols·Robert D CardiffRobert J Munn
Aug 6, 2014·Research in Veterinary Science·Niladri MukherjeeSanti P Sinha Babu
Dec 3, 2014·Asian Pacific Journal of Tropical Medicine·Niladri MukherjeeSanti P Sinha Babu
Feb 24, 2015·Bioscience Reports·Devis PascutClaudio Tiribelli
Nov 21, 2015·Mini Reviews in Medicinal Chemistry·Niladri MukherjeeSanti P Sinha Babu
Feb 7, 2016·Free Radical Biology & Medicine·Niladri MukherjeeSanti P Sinha Babu
Citations
Dec 7, 2018·PloS One·Anindya Sundar RaySanti P Sinha Babu
May 21, 2019·Parasitology·Priya RoySanti P Sinha Babu
Sep 16, 2019·International Journal of Biological Macromolecules·Nikhilesh Joardar, Santi P Sinha Babu
Apr 26, 2021·Free Radical Biology & Medicine·Nikhilesh JoardarSanti P Sinha Babu
May 1, 2021·3 Biotech·Subendu SarkarGorachand Bhattacharya