Antifreeze Glycoproteins Bind Irreversibly to Ice

Journal of the American Chemical Society
Konrad MeisterRan Drori

Abstract

Antifreeze proteins (AFPs) and antifreeze glycoproteins (AFGPs) inhibit ice growth via an adsorption-inhibition mechanism that assumes irreversible binding of AF(G)Ps to embryonic ice crystals and the inhibition of further growth. The irreversible binding of antifreeze glycoproteins (AFGPs) to ice has been questioned and remains poorly understood. Here, we used microfluidics and fluorescence microscopy to investigate the nature of the binding of small and large AFGP isoforms. We found that both AFGP isoforms bind irreversibly to ice, as evidenced by microfluidic solution exchange experiments. We measured the adsorption rate of the large AFGP isoform and found it to be 50% faster than that of AFP type III. We also found that the AFGP adsorption rate decreased by 65% in the presence of borate, a well-known inhibitor of AFGP activity. Our results demonstrate that the adsorption rate of AFGPs to ice is crucial for their ice growth inhibition capability.

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Citations

Feb 1, 2019·Physical Chemistry Chemical Physics : PCCP·Poonam Pandey, Sairam S Mallajosyula
Apr 16, 2019·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Yoshinori FurukawaGen Sazaki
Oct 20, 2019·International Journal of Molecular Sciences·Romà Surís-Valls, Ilja K Voets
Feb 15, 2020·Biomolecules·Aneta BiałkowskaAleksandra Twarda-Clapa
Sep 7, 2020·The Journal of Chemical Physics·Valentino BiancoCarlos Vega
Jul 28, 2020·Critical Reviews in Food Science and Nutrition·Xu ChenShaoyun Wang
May 16, 2019·Macromolecular Bioscience·Caroline I BiggsMatthew I Gibson
Nov 12, 2019·Journal of the American Chemical Society·Tehilla BergerRan Drori
Jan 15, 2021·Comprehensive Reviews in Food Science and Food Safety·Xu ChenShaoyun Wang
Nov 25, 2020·The Journal of Physical Chemistry. B·Jinzi DengRan Drori
May 7, 2021·Biomacromolecules·Yuling SunKonrad Meister
Sep 18, 2018·The Journal of Physical Chemistry. B·Uday Sankar Midya, Sanjoy Bandyopadhyay
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Jan 8, 2019·The Journal of Physical Chemistry Letters·Giulia GiubertoniHuib J Bakker
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Aug 19, 2021·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Tamar Nicole SoussanaRan Drori
Dec 21, 2021·Biomacromolecules·Kartik KamatBaron Peters

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