Antifungal Activities of Volatile Secondary Metabolites of Four Diaporthe Strains Isolated from Catharanthus roseus

Journal of Fungi
Dong-Hui YanGary Strobel

Abstract

Four endophytic fungi were isolated from the medicinal plant, Catharanthus roseus, and were identified as Diaporthe spp. with partial translation elongation factor 1-alpha (TEF1), beta-tubulin (TUB), histone H3 (HIS), calmodulin (CAL) genes, and rDNA internal transcribed spacer (ITS) region (TEF1-TUB-HIS--CAL-ITS) multigene phylogeny suggested for species delimitation in the Diaporthe genus. Each fungus produces a unique mixture of volatile organic compounds (VOCs) with an abundant mixture of terpenoids analyzed by headspace solid-phase microextraction (SPME) fiber-GC/MS. These tentatively-detected terpenes included α-muurolene, β-phellandrene, γ-terpinene, and α-thujene, as well as other minor terpenoids, including caryophyllene, patchoulene, cedrene, 2-carene, and thujone. The volatile metabolites of each isolate showed antifungal properties against a wide range of plant pathogenic test fungi and oomycetes, including Alternaria alternata, Botrytis cinerea, Colletotrichum gloeosporioides, Fusarium graminearum, and Phytophthora cinnamomi. The growth inhibition of the pathogens varied between 10% and 60% within 72 h of exposure. To our knowledge, the endophytic Diaporthe-like strains are first reported from Catharanthus roseus. ...Continue Reading

References

Apr 1, 1995·Applied and Environmental Microbiology·N L Glass, G C Donaldson
Nov 9, 2001·Microbiology·G A StrobelC Markworth
Feb 3, 2009·Journal of Pharmaceutical and Biomedical Analysis·P Guedes De PinhoMariana Sottomayor
Jan 7, 2010·BMC Bioinformatics·Ben C Stöver, Kai F Müller
Apr 25, 2012·Canadian Journal of Microbiology·Erick Francisco RakotonirianaJoëlle Quetin-Leclercq
Jan 19, 2013·Molecular Biology and Evolution·Kazutaka Katoh, Daron M Standley
Jun 6, 2013·Journal of Natural Products·Samuel BertrandJean-Luc Wolfender
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Mar 4, 2014·TheScientificWorldJournal·Dhanya N Nair, S Padmavathy
Jul 6, 2014·Current Opinion in Microbiology·Gary Strobel
Jun 1, 2016·Microbiological Research·Paulo José Camargo Dos SantosChirlei Glienke
Jun 9, 2016·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·Farah Wahida Ayob, Khanom Simarani
Jan 17, 2017·Fungal Biology·Mário Cesar Jucoski BierCarlos Ricardo Soccol
Feb 6, 2017·FEMS Microbiology Letters·Fabiana TonialChirlei Glienke
Feb 17, 2017·Natural Product Reports·Jeroen S Dickschat
Jun 7, 2017·Journal of the Science of Food and Agriculture·Ram S VermaManish K Kandwal
Nov 22, 2017·Current Microbiology·Chutima TanapichatsakulPatcharee Pripdeevech

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

Mega
PAUP
TreeGraph
ABI Peak Scanner
SequenceMatrix
PartitionFinder
BioEdit
MAFFTv
Treebase

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.