Antifungal activity of chitinase II against Colletotrichum falcatum Went. causing red rot disease in transgenic sugarcane

Turkish Journal of Biology = Türk Biyoloji Dergisi
Muhammad TariqTayyab Husnain

Abstract

We evaluated transgenic lines of sugarcane modified with the barley chitinase class-II gene to create resistance against the red rot causative agent Colletotrichum falcatum Went. Local sugarcane cultivar SP93 was transformed with a 690-bp coding sequence of the chitinase-II gene under the influence of a polyubiquitin promoter. Transgenic sugarcane lines (T 0) overexpressing the chitinase gene were obtained through a particle bombardment method with 13.3% transformation efficiency. Four transgenic sugarcane lines, SCT-03, SCT-05, SCT-15, and SCT-20, were tested for resistance against red rot by in vitro antifungal assays. Crude protein extracts from transgenic sugarcane plants SCT-03, SCT-05, SCT-15, and SCT-20 inhibited the mycelial growth of C. falcatum by 49%, 40%, 56%, and 52%, respectively, in a quantitative in vitro assay. Our findings revealed that two transgenic lines, SCT-15 and SCT-20, exhibited the highest endochitinase activity of 0.72 and 0.58 U/mL, respectively. Furthermore, transgenic lines SCT-15 and SCT-20 exhibited strong resistance against inoculated C. falcatum in an in vitro bioassay, as they remained healthy and green in comparison with the control sugarcane plants, which turned yellow and eventually died 3...Continue Reading

References

Jun 9, 2006·Plant Cell Reports·Young Hee Joung, Kathryn Kamo
Oct 13, 2006·Protein Expression and Purification·S Isaac Kirubakaran, N Sakthivel
May 10, 2008·Journal of Experimental Botany·Sanghyun ShinGary J Muehlbauer
Jun 4, 2015·Scientific Reports·Yachun SuYouxiong Que
Feb 22, 2017·Genetics and Molecular Biology·Thais P SouzaMarcio C Silva-Filho

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Citations

Oct 18, 2020·Functional & Integrative Genomics·Uilian Stefanello de MelloMárcio Henrique Pereira Barbosa
Nov 23, 2020·Molecular Biology Reports·Ibrahim Ilker Ozyigit, Kuaybe Yucebilgili Kurtoglu
Aug 25, 2020·Microbial Pathogenesis·Maria Swiontek BrzezinskaUrszula Jankiewicz
Jan 26, 2022·Journal of Basic Microbiology·Bhoomika M KaramchandaniSurekha K Satpute

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