Antifungal compounds from Melia azedarach leaves for management of Ascochyta rabiei, the cause of chickpea blight

Natural Product Research
Khajista JabeenMakshoof Athar

Abstract

The antifungal activity of Melia azedarach L. leaves was investigated against Ascochyta rabiei (Pass.) Lab., the cause of destructive blight disease of chickpea (Cicer arietinum L.). Bioassay guided fractionation revealed that the chloroform fraction of the methanolic extract of M. azedarach leaves was highly effective against A. rabiei. Six compounds, namely β-sitosterol (1), β-amyrin (2), ursolic acid (3), benzoic acid (4), 3,5 dimethoxybenzoic acid (5) and maesol (6) were isolated from the chloroform fraction through column chromatography. The in vitro antifungal activity of compounds 2-5 was evaluated against A. rabiei. A commercial fungicide, mancozeb, was used as a positive control. Different concentrations of mancozeb and the isolated compounds, ranging from 0.0039 to 4 mg mL(-1), were used in the antifungal bioassay, and data regarding minimum inhibitory concentration (MIC) was recorded 24, 48 and 72 h after incubation. All concentrations of mancozeb inhibited the fungal spore germination at all three incubation periods. The tested compounds exhibited variable antifungal activity against the target fungal pathogens. All compounds showed their highest antifungal activity after 24 h of incubation. Compound 2 was found to ...Continue Reading

References

May 26, 2005·Journal of Agricultural and Food Chemistry·Raquel Rial-OteroJesús Simal-Gándara
Mar 17, 2007·Natural Product Research·Ejaz AhmedMuhammad Iqbal Choudhary
Jul 12, 2008·Biotechnology Advances·Ahmad Ali ShahidSheikh Riazuddin

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Citations

Oct 19, 2011·Natural Product Research·Arshad Javaid, Amna Saddique
Nov 18, 2018·Natural Product Research·Jin ZengQin-Gang Tan
Jun 23, 2020·Journal of Biomolecular Structure & Dynamics·Tanuja JoshiSubhash Chandra
Jun 6, 2018·Frontiers in Pharmacology·Annadurai SenthilkumarAbdul J Cheruth
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Pooja SharmaSuresh Kumar

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Methods Mentioned

BETA
column chromatography

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An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

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