Antifungal mechanisms of a plant defensin MtDef4 are not conserved between the ascomycete fungi Neurospora crassa and Fusarium graminearum

Molecular Microbiology
Kaoutar El-MounadiDilip M Shah

Abstract

Defensins play an important role in plant defense against fungal pathogens. The plant defensin, MtDef4, inhibits growth of the ascomycete fungi, Neurospora crassa and Fusarium graminearum, at micromolar concentrations. We have reported that MtDef4 is transported into the cytoplasm of these fungi and exerts its antifungal activity on intracellular targets. Here, we have investigated whether the antifungal mechanisms of MtDef4 are conserved in these fungi. We show that N. crassa and F. graminearum respond differently to MtDef4 challenge. Membrane permeabilization is required for the antifungal activity of MtDef4 against F. graminearum but not against N. crassa. We find that MtDef4 is targeted to different subcellular compartments in each fungus. Internalization of MtDef4 in N. crassa is energy-dependent and involves endocytosis. By contrast, MtDef4 appears to translocate into F. graminearum autonomously using a partially energy-dependent pathway. MtDef4 has been shown to bind to the phospholipid phosphatidic acid (PA). We provide evidence that the plasma membrane localized phospholipase D, involved in the biosynthesis of PA, is needed for entry of this defensin in N. crassa, but not in F. graminearum. To our knowledge, this is th...Continue Reading

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Citations

Jun 19, 2017·Protein Expression and Purification·Francisco Guillén-ChableGeorgina Estrada
Feb 9, 2018·Journal of Fungi·Brigitte M E HayesNicole L van der Weerden
Aug 14, 2018·Journal of Experimental Botany·Marcelo Lattarulo CamposOctávio Luiz Franco
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Jun 20, 2019·Protein and Peptide Letters·Kaushik DasSwapan K Datta
Jun 21, 2020·BMC Microbiology·Andrew E SathoffDeborah A Samac
Jan 23, 2020·Journal of Proteome Research·Thomas AumerPhilippe Bulet
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Nov 12, 2017·International Journal of Food Microbiology·Thibaut TheryElke K Arendt
Apr 30, 2021·Frontiers in Cell and Developmental Biology·Caroline StruyfsKarin Thevissen

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