Abstract
Two antifungal peptides (designated alpha- and beta-basrubrins) with molecular masses of 4-5 kDa and distinct N-terminal sequences, and a peptide and a protein with N-terminal sequences resembling heat shock protein (hsp) and serine-threonine kinase, respectively, were isolated from seeds of the Ceylon spinach Basella rubra. The purification procedure entailed saline extraction, (NH4)2SO4 precipitation, ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on CM-cellulose, and FPLC-gel filtration on a Superdex peptide column. alpha- and beta-basrubrins inhibited mycelial growth in Botrytis cirerea with an IC50 value of 7.5 and 14.7 microM, respectively, Mycosphaerella arachidicola with an IC50 of 12.4 and 6.9 microM, and Fusarium oxysporum with an IC50 of 5.8 and 6.2 microM. Neither alpha-basrubrin nor beta-basrubin exhibited DNase, RNase, lectin or protease activity, indicating that their antifungal action is not due to these activities. HIV-1 reverse transcriptase was inhibited by alpha- and beta-basrubrins with an IC50 of 246 and 370 microM, respectively. Translation in rabbit reticulocyte lysate was inhibited by alpha- and beta-basrubrins with an IC50 of 400...Continue Reading
References
Nov 15, 1973·Journal of Molecular Biology·U K Laemmli, M Favre
Mar 1, 1994·Plant Physiology·M DasGupta
Jun 1, 1996·Plant Molecular Biology·C P Joshi, H T Nguyen
Mar 1, 1996·Immunopharmacology·H X WangS T Chang
Nov 1, 1996·Plant Physiology·J H BarkerN G Halford
Jan 1, 1997·Life Sciences·R A CollinsH W Yeung
Aug 5, 1998·Pharmacology & Therapeutics·A R Kennedy
Nov 11, 1999·Plant Physiology·R CarrancoJ Jordano
Mar 1, 2000·Biochemical and Biophysical Research Communications·X Y YeT B Ng
Apr 12, 2000·Plant Physiology·N Wehmeyer, E Vierling
Jul 13, 2000·Biochemical and Biophysical Research Communications·X Y Ye, T B Ng
Dec 19, 2000·Biochemical and Biophysical Research Communications·H Wang, T B Ng
May 1, 2001·The Journal of Peptide Research : Official Journal of the American Peptide Society·X Y Ye, T B Ng
Nov 2, 2001·Biochemical and Biophysical Research Communications·H Wang, T B Ng
Oct 9, 2002·Current Protein & Peptide Science·T Fukamizo
May 29, 2003·Journal of Insect Physiology·M A. AndersonR L. Heath
Oct 1, 1992·Plant Physiology·J S Marshall, K Keegstra
Aug 1, 1991·Plant Physiology·P S DietrichR M Sinibaldi
Citations
May 18, 2005·The International Journal of Biochemistry & Cell Biology·Jack Ho Wong, Tzi Bun Ng
May 4, 2010·Journal of Ethnopharmacology·Nisarat SiriwatanametanonMichael Heinrich
Jun 14, 2005·Peptides·Jack Ho Wong, Tzi Bun Ng
Nov 5, 2005·Peptides·Jack Ho Wong, Tzi Bun Ng
Apr 2, 2009·Bioscience Reports·Peng LinTzi Bun Ng
Jul 23, 2013·Food Chemistry·Peng LinLixin Xia
Aug 16, 2018·EMBO Molecular Medicine·Johanna KondelinLauri A Aaltonen