Abstract
The objective of the present study was to isolate Actinobacteria, preferably Streptomyces spp. from the rhizosphere soils of three ethno-medicinal plants collected in Serbia (Papaver rhoeas, Matricaria chamomilla, and Urtica dioica) and to screen their antifungal activity against Candida spp. Overall, 103 sporulating isolates were collected from rhizosphere soil samples and determined as Streptomyces spp. Two different media and two extraction procedures were used to facilitate identification of antifungals. Overall, 412 crude cell extracts were tested against Candida albicans using disk diffusion assays, with 42% (43/103) of the strains showing the ability to produce antifungal agents. Also, extracts inhibited growth of important human pathogens: Candida krusei, Candida parapsilosis, and Candida glabrata. Based on the established degree and range of antifungal activity, nine isolates, confirmed as streptomycetes by 16S rRNA sequencing, were selected for further testing. Their ability to inhibit Candida growth in liquid culture, to inhibit biofilm formation, and to disperse pre-formed biofilms was assessed with active concentrations from 8 to 250 μg/mL. High-performance liquid chromatographic profiles of extracts derived from s...Continue Reading
References
Jan 1, 1992·International Journal of Systematic Bacteriology·V HájekM Kocur
Aug 30, 1984·Mycopathologia·A A Kostiala, I Kostiala
Jun 1, 1994·Applied and Environmental Microbiology·A L ReysenbachN R Pace
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Sep 6, 2002·Microbiology·Uvidelio F CastilloDebbie Yaver
Sep 27, 2002·Chembiochem : a European Journal of Chemical Biology·Helge Björn BodeAxel Zeeck
Apr 15, 2003·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M F VicenteF Peláez
Apr 15, 2003·Nature Biotechnology·Haruo IkedaSatoshi Omura
Jul 3, 2003·Research in Microbiology·Lotfi MellouliSamir Bejar
Aug 2, 2003·FEMS Microbiology Letters·Uvidelio CastilloDavid Teplow
Sep 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Gregory L Challis, David A Hopwood
Nov 25, 2003·BioTechniques·Jasmina NikodinovicJo-Anne Chuck
Jun 21, 2005·Journal of Ethnopharmacology·J L Ríos, M C Recio
Oct 1, 1943·Journal of Bacteriology·S A Waksman, A T Henrici
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Jun 28, 2011·Applied Microbiology and Biotechnology·Lidija DjokicBranka Vasiljevic
Aug 3, 2012·BMC Microbiology·Silvia D SchreyMika T Tarkka
Aug 15, 2013·Journal of Applied Microbiology·N StankovicJ Nikodinovic-Runic
Oct 29, 2013·BioImpacts : BI·Hadi MalekiSajjad Khani
Jan 7, 2014·Frontiers in Microbiology·Martina KöberlGabriele Berg
Jan 17, 2014·Microbiology·Rahul JogShalini Rajkumar
Feb 13, 2014·Letters in Applied Microbiology·A VartakG B Mahajan
Feb 1, 2015·Applied Microbiology and Biotechnology·Tatjana Ilic-TomicNiko S Radulović
Jun 22, 2015·Antonie van Leeuwenhoek·Patrycja GolinskaMahendra Rai
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Mar 25, 2017·Journal of Natural Products·Max CrüsemannBradley S Moore
Aug 19, 2017·Natural Product Reports·Olga Genilloud
May 26, 2018·Biotechnology and Applied Biochemistry·Jelena SpasicLidija Djokic