Antigen analysis of Mycobacterium tuberculosis H37Rv culture filtrate proteins

Scandinavian Journal of Immunology
Hiwa MålenHarald G Wiker

Abstract

Culture filtrates of Mycobacterium tuberculosis H37Rv highly enriched with secreted proteins were used to identify antigens recognized by a serum pool from tuberculosis patients. Two different approaches were used to separate the culture filtrate protein mixture: (i) proteins were fractionated according to their hydrophobicity using an HPLC-C18 chromatography column followed by separation based on their molecular mass by SDS-PAGE and subsequent immunoblotting or (ii) proteins were separated by two-dimensional gel electrophoresis, based on their isoelectric point and their molecular mass. Twenty serologically reactive proteins were ultimately identified by both methods, including four novel antigens. Further, to estimate the immunogenicity of the identified culture filtrate proteins, the relative antibody quantities were measured using Image master software. Our results show that the antibodies against proteins belonging to the antigen 85 complex were the most abundant in the serum of patients with active tuberculosis. The most immunogenic proteins in terms of high antibody-to-protein-ratio were Rv3881c and three lipoproteins Rv0934 (the 38 kDa antigen), Rv0932c (pstS2), and Rv3006 (LppZ). Rv 3881c [corrected] is located close t...Continue Reading

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Citations

Feb 2, 2011·Immunology and Cell Biology·Veerle De ColvenaerGeorges Leclercq
Jan 25, 2013·Molecular & Cellular Proteomics : MCP·Jakob AlbrethsenIda Rosenkrands
Jan 15, 2014·Diagnostic Microbiology and Infectious Disease·Ponrut PhunpaeWatchara Kasinrerk
Apr 10, 2008·Scandinavian Journal of Immunology·D K Kaushik, D Sehgal
Jul 5, 2011·Proteomics·Gustavo A de Souza, Harald G Wiker
Aug 5, 2015·Journal of Proteomics·Manju LataKrishnamurthy Venkatesan
Jan 24, 2014·Pathogens and Disease·Patrick W KernsMark E Shirtliff
Jul 28, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gayathri NagalingamBernadette M Saunders
Dec 19, 2017·Frontiers in Cellular and Infection Microbiology·Jia-Ni XiaoYing Wang
Aug 30, 2019·Journal of Immunoassay & Immunochemistry·Kantinan ChuensirikulchaiWatchara Kasinrerk
Sep 10, 2010·Journal of Proteome Research·Lisa M WolfeKaren M Dobos

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